Sakoui Souraya, Derdak Reda, Pop Oana Lelia, Vodnar Dan Cristian, Addoum Boutaina, Teleky Bernadette-Emőke, Elemer Simon, Elmakssoudi Abdelhakim, Suharoschi Ramona, Soukri Abdelaziz, El Khalfi Bouchra
Laboratory of Physiopathology, Molecular Genetics & Biotechnology, Faculty of Sciences Ain Chock, Health and Biotechnology Research Centre, Hassan II University of Casablanca, Maarif B.P, 5366, Casablanca, Morocco.
Department of Food Science, University of Agricultural Science and Veterinary Medicine, 3-5 Calea Mănăștur, 400372, Cluj-Napoca, Romania.
Curr Res Food Sci. 2022 Sep 30;5:1713-1719. doi: 10.1016/j.crfs.2022.09.027. eCollection 2022.
In the current research, SRBG1 newly isolated from Bat guano was encapsulated using spray drying technique to create a probiotic powder using six combinations of inulin, maltodextrin and sodium alginate. The encapsulation yield, moisture content, physical characteristics, and shape were investigated. Microcapsules yields ranged from 67 to 85 percent, which is consistent with typical B-290 spray-drier yields. The moisture content showed to increase (4 ± 0.15%) with the addition of sodium alginate to inulin and maltodextrin. In the gastrointestinal conditions (simulated gastric juice and bile salts), it was shown that the viability of probiotic cells in capsules was higher than that of free cells. This demonstrated the effectiveness of combining inulin and maltodextrin to encapsulate substances in surviving in gastro-intestinal conditions. Additionally, we evaluated the non-encapsulated and encapsulated SRBG1 by assessing their impact on the rheological parameters of fermented milk. The results showed that in the absence of sodium alginate the viscosity of milk was lower than with the other protectors, which was confirmed by the quick acidification of the fermented milk by microcapsules containing sodium alginate.
在当前的研究中,采用喷雾干燥技术对从蝙蝠粪便中新分离出的SRBG1进行包封,使用菊粉、麦芽糊精和海藻酸钠的六种组合制备益生菌粉末。研究了包封率、水分含量、物理特性和形状。微胶囊产率在67%至85%之间,这与典型的B - 290喷雾干燥器产率一致。随着海藻酸钠添加到菊粉和麦芽糊精中,水分含量有所增加(4 ± 0.15%)。在胃肠道条件(模拟胃液和胆汁盐)下,结果表明胶囊中益生菌细胞的活力高于游离细胞。这证明了菊粉和麦芽糊精组合在胃肠道条件下对包封物质存活的有效性。此外,我们通过评估未包封和包封的SRBG1对发酵乳流变学参数的影响来进行评价。结果表明,在没有海藻酸钠的情况下,牛奶的粘度低于使用其他保护剂时的粘度,这通过含海藻酸钠的微胶囊使发酵乳快速酸化得到证实。