Department of Dentistry, Dijlah University College, Baghdad, Iraq.
Department of Oral Diagnosis, College of Dentistry, University of Baghdad, Baghdad, Iraq.
Pan Afr Med J. 2022 Jul 7;42:186. doi: 10.11604/pamj.2022.42.186.35429. eCollection 2022.
pemphigus vulgaris (PV) is an autoimmune condition characterized by the loss of adhesion between the epithelial cells and blister formation. The production of autoantibodies against desmosomal proteins, namely, desmoglein (DSG) 1 and DSG3, is considered a main event of PV. A full understanding of the role of adhesion molecules in the pathogenesis of PV is not fully elucidated yet. This study aimed to evaluate and correlate the immunohistochemical expression of E-cadherin (E-cad), DSG1, and DSG3 proteins in oral and skin PV.
this study was a retrospective analysis study. Positive PV cases were stained with anti-E-cad, anti-DSG1, and anti-DSG3 antibodies. The expression of each marker was determined by two pathologists according to an established scoring system: (E-cad: negative, weak, moderate, and strong), (DSG1: negative, weak, and strong), and (DSG3: negative and positive). The Chi-square and Pearson´s correlation tests were used to statistically analyze the data.
forty-three biopsies (26 skin and 17 oral tissue samples) from 22 males and 21 female PV patients were included. The median age was 40.50 years. In total, the immunohistochemical expression was negative for DSG3, E-cad, and DSG1 in 81.4%, 18.5%, and 16.4%, respectively. DSG1 expression was significantly higher in males than females. A statistically significant correlation was found between E-cad and DSG3 expressions.
a significant difference in the expression of markers of both oral and skin PV was absent. Downregulation of DSG3 expression was the hallmark feature that also showed a positive correlation with E-cad expression.
寻常型天疱疮（PV）是一种自身免疫性疾病，其特征是上皮细胞间黏附丧失和水疱形成。抗桥粒芯糖蛋白（DSG）1 和 DSG3 等桥粒蛋白的自身抗体的产生被认为是 PV 的主要事件。然而，黏附分子在 PV 发病机制中的作用尚未完全阐明。本研究旨在评估和比较口腔和皮肤 PV 中 E-钙黏蛋白（E-cad）、DSG1 和 DSG3 蛋白的免疫组织化学表达，并进行相关性分析。
本研究为回顾性分析研究。使用抗 E-cad、抗 DSG1 和抗 DSG3 抗体对阳性 PV 病例进行染色。根据既定评分系统，由两位病理学家确定每个标志物的表达：（E-cad：阴性、弱阳性、中度阳性和强阳性）、（DSG1：阴性、弱阳性和强阳性）和（DSG3：阴性和阳性）。采用卡方检验和 Pearson 相关性检验对数据进行统计学分析。
共纳入 22 名男性和 21 名女性 PV 患者的 43 份活检标本（26 份皮肤和 17 份口腔组织样本），中位年龄为 40.50 岁。总的来说，DSG3、E-cad 和 DSG1 的免疫组织化学表达分别为 81.4%、18.5%和 16.4%阴性。DSG1 的表达在男性中明显高于女性。E-cad 和 DSG3 的表达之间存在统计学显著相关性。
口腔和皮肤 PV 中标志物的表达无明显差异。DSG3 表达下调是一个显著特征，与 E-cad 表达呈正相关。
