He Fei, Zhou Xiongming, Huang Gan, Jiang Qingkun, Wan Li, Qiu Jiaxuan
Department of Stomatology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi, China.
J Oncol. 2022 Sep 5;2022:3135470. doi: 10.1155/2022/3135470. eCollection 2022.
Oral squamous cell carcinoma is the most common head and neck malignancy with high morbidity and mortality. Currently, platinum-based chemotherapy is the conventional chemotherapy regimen for patients with oral squamous cell carcinoma. However, due to the heterogeneity of tumors and individual differences of patients, chemotherapy regimens lacking individualized evaluation of tumor patients are often less effective. Therefore, personalized tumor chemotherapy is one of the effective methods for the future treatment of malignant tumors. The patient-derived xenograft model is a relatively new tumor xenograft model that relies on immunodeficient mice. This model can better maintain various histological characteristics of primary tumor grafts, such as pathological structural features, molecular diversity, and gene expression profiles. Therefore, the patient-derived xenograft model combined with drug screening technology to explore new tumor chemotherapy is the critical research direction for future tumor treatment. This study successfully established the patient-derived xenograft model of oral squamous cell carcinoma. It was verified by hematoxylin-eosin staining and immunohistochemistry that the constructed patient-derived xenograft model retained the pathological and molecular biological characteristics of primary tumors. Our patient-derived xenograft model can be used further to study the oncological characteristics of oral squamous carcinoma and can also be applied to personalize the treatment of oral squamous carcinoma patients, providing a practical resource for screening chemotherapy drugs.
口腔鳞状细胞癌是最常见的头颈部恶性肿瘤，发病率和死亡率都很高。目前，铂类化疗是口腔鳞状细胞癌患者的传统化疗方案。然而，由于肿瘤的异质性和患者的个体差异，缺乏对肿瘤患者个体化评估的化疗方案往往效果较差。因此，个性化肿瘤化疗是未来恶性肿瘤治疗的有效方法之一。患者来源的异种移植模型是一种相对较新的肿瘤异种移植模型，它依赖于免疫缺陷小鼠。该模型能够更好地保持原发性肿瘤移植物的各种组织学特征，如病理结构特征、分子多样性和基因表达谱。因此，将患者来源的异种移植模型与药物筛选技术相结合来探索新的肿瘤化疗方法是未来肿瘤治疗的关键研究方向。本研究成功建立了口腔鳞状细胞癌患者来源的异种移植模型。通过苏木精-伊红染色和免疫组织化学验证，所构建的患者来源的异种移植模型保留了原发性肿瘤的病理和分子生物学特征。我们的患者来源的异种移植模型可进一步用于研究口腔鳞状细胞癌的肿瘤学特征，也可应用于口腔鳞状细胞癌患者的个性化治疗，为筛选化疗药物提供实用资源。
