Departamento de Fisiología y Farmacología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Ciudad Universitaria, Aguascalientes, Mexico.
Departamento de Fisiología y Farmacología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Ciudad Universitaria, Aguascalientes, Mexico;
Acta Neurobiol Exp (Wars). 2022;82(3):304-314. doi: 10.55782/ane-2022-029.
GPR55 is an orphan receptor whose endogenous agonists include lysophosphatidylinositol (LPI) and N‑acetylethanolamides (NAEs), such as palmitoylethanolamide (PEA) and anandamide. Furthermore, its physiology in the central nervous system involves motor coordination, procedural and spatial memory, pain, and anxiety, among others. Recent reports indicate that systemic injections of O‑1602 (a GPR55 and GPR18 agonist) blocked the reinforcing effects of morphine and nicotine in the conditioned place preference (CPP) paradigm, suggesting a possible participation of peripheral and/or central GPR55/GPR18 in brain reward/anti‑reward systems. In this pilot study, the endogenous GPR55 agonists LPI and PEA, the highly selective GPR55 synthetic agonist ML184 or the selective GPR55 antagonist ML193 were injected to examine their pharmacological effects on the reinforcing actions of nicotine in the CPP paradigm. Our preliminary study shows that injections of LPI, PEA, ML184 and ML193 interfered with the change in place preference induced by nicotine via mechanisms that remain to be identified (which probably include central GPR55).
GPR55 是一种孤儿受体，其内源性激动剂包括溶血磷脂酰肌醇（LPI）和 N-乙酰乙醇胺（NAE），如棕榈酰乙醇酰胺（PEA）和大麻素。此外，其在中枢神经系统中的生理学涉及运动协调、程序性和空间记忆、疼痛和焦虑等。最近的报告表明，全身性注射 O-1602（GPR55 和 GPR18 激动剂）阻断了条件位置偏好（CPP）范式中吗啡和尼古丁的强化作用，这表明外周和/或中枢 GPR55/GPR18 可能参与了大脑奖励/抗奖励系统。在这项初步研究中，内源性 GPR55 激动剂 LPI 和 PEA、高选择性 GPR55 合成激动剂 ML184 或选择性 GPR55 拮抗剂 ML193 被注射，以检查它们对 CPP 范式中尼古丁强化作用的药理学影响。我们的初步研究表明，LPI、PEA、ML184 和 ML193 的注射通过尚未确定的机制干扰了尼古丁引起的位置偏好的变化（可能包括中枢 GPR55）。
