Center for Advanced Reproductive Services, Division of Reproductive Endocrinology & Infertility, University of Connecticut Health Center, 2 Batterson Park Rd, Farmington, CT, 06032, USA.
Reproductive Medicine Associates of Philadelphia, 1015 Chestnut Street, 8th Floor, Philadelphia, PA, 19107, USA.
J Assist Reprod Genet. 2022 Nov;39(11):2521-2528. doi: 10.1007/s10815-022-02624-8. Epub 2022 Oct 10.
The purpose of this study was to determine the impact of body mass index (BMI) on euploidy rates for in vitro fertilization (IVF) cycles with preimplantation genetic testing (PGT) utilizing primarily next-generation sequencing (NGS).
This retrospective cohort study included women aged ≤ 45 years who underwent IVF/PGT between September 2013 and September 2020 at a single university-affiliated fertility center. The primary outcome was euploidy rate. Secondary outcomes included peak serum estradiol (E2), number of oocytes retrieved, oocyte maturation rate, high-quality blastulation rate, clinical loss rate (CLR), clinical pregnancy rate (CPR), and ongoing pregnancy/live birth rate (OPR/LBR).
The study included 1335 IVF cycles that were stratified according to BMI (normal, n = 648; overweight, n = 377; obese, n = 310). The obese group was significantly older with significantly lower baseline FSH, peak E2, high-quality blastulation rate, and number of embryos biopsied than the normal group. Overall euploidy rates were not significantly different between BMI groups (normal 36.4% ± 1.3; overweight 37.3% ± 1.8; obese 32.3% ± 1.8; p = 0.11), which persisted after controlling for covariates (p = 0.82) and after stratification of euploidy rate by age group and by number of oocytes retrieved per age group. There were no significant differences in CLR, CPR, and OPR/LBR across BMI groups.
Despite a lower high quality blastulation rate with obesity, there is not a significant difference in euploidy rates across BMI groups in women undergoing IVF/PGT.
本研究旨在确定体质量指数（BMI）对利用新一代测序（NGS）进行的体外受精（IVF）-胚胎植入前遗传学检测（PGT）周期中整倍体率的影响。
本回顾性队列研究纳入了 2013 年 9 月至 2020 年 9 月在一家大学附属生育中心接受 IVF/PGT 的年龄≤45 岁的女性。主要结局是整倍体率。次要结局包括血清雌二醇（E2）峰值、获卵数、卵母细胞成熟率、优质胚胎囊胚形成率、临床流产率（CLR）、临床妊娠率（CPR）和活产率（OPR/LBR）。
研究纳入了 1335 个 IVF 周期，根据 BMI（正常 BMI，n=648；超重 BMI，n=377；肥胖 BMI，n=310）进行分层。肥胖组的年龄明显较大，基础 FSH、E2 峰值、优质胚胎囊胚形成率和活检胚胎数明显较低。BMI 各组间的整倍体率无显著差异（正常 BMI 组为 36.4%±1.3%；超重 BMI 组为 37.3%±1.8%；肥胖 BMI 组为 32.3%±1.8%；p=0.11），调整协变量后（p=0.82）和按年龄组和每个年龄组的获卵数分层后，整倍体率仍无显著差异。BMI 各组间的 CLR、CPR 和 OPR/LBR 无显著差异。
尽管肥胖组优质胚胎囊胚形成率较低，但 BMI 各组间 IVF/PGT 女性的整倍体率无显著差异。
