Baser Emre, Inandiklioglu Nihal, Aydogan Kırmızı Demet, Ercan Fedi, Caniklioğlu Ayşen, Kara Mustafa, Onat Taylan, Yalvac Ethem Serdar
Department of Obstetrics and Gynecology, Yozgat Bozok University, Yozgat, Turkey.
Medical Biology, Bozok University Faculty of Medicine, Yozgat, Turkey.
Z Geburtshilfe Neonatol. 2023 Apr;227(2):112-119. doi: 10.1055/a-1938-0010. Epub 2022 Oct 10.
Although the etiopathogenesis of preeclampsia (PE) is unknown, evidence suggests that it may be associated with increased oxidative stress. Studies have shown that oxidative stress can affect DNA fragments called telomeres. However, the interactions of PE, oxidative stress, and telomere length are not clearly known. This study aims to evaluate the oxidative/anti-oxidative stress balance in the placenta and umbilical cord and examine the effect of oxidative stress on telomeres.
MATERIALS-METHOD: Cord blood and placental samples were collected from 27 pregnant women with severe PE (28-33 gestational weeks) and 53 healthy pregnant women. Telomere length (TL) was measured by real-time PCR in the cord blood and placenta tissue. Total antioxidant status (TAS) and total oxidant status (TOS) levels were measured in the cord blood and placenta tissue using a colorimetric method.
No significant differences were found between groups regarding age, BMI, gravida, parity, and newborn gender (p>0.05). Cord blood and placental TL of PE patients were significantly shorter than the control group, while cord blood and placental TAS and TOS levels were higher (p<0.05). The results of a multivariate logistic regression analysis showed that the level of placental TOS in PE patients (OR=1.212, 95% CI=1.068-1.375) was an independent risk factor affecting PE.
This study found that oxidative stress is an independent risk factor in the development of PE and shortens TL in both placental and umbilical cord blood. Future research on telomere homeostasis may offer a new perspective for the treatment of PE.
尽管子痫前期（PE）的发病机制尚不清楚，但有证据表明其可能与氧化应激增加有关。研究表明，氧化应激可影响称为端粒的DNA片段。然而，PE、氧化应激和端粒长度之间的相互作用尚不清楚。本研究旨在评估胎盘和脐带中的氧化/抗氧化应激平衡，并研究氧化应激对端粒的影响。
收集27例重度PE孕妇（孕28 - 33周）和53例健康孕妇的脐血和胎盘样本。采用实时PCR法测量脐血和胎盘组织中的端粒长度（TL）。采用比色法测量脐血和胎盘组织中的总抗氧化状态（TAS）和总氧化状态（TOS）水平。
两组在年龄、BMI、孕周、产次和新生儿性别方面无显著差异（p>0.05）。PE患者的脐血和胎盘TL显著短于对照组，而脐血和胎盘的TAS和TOS水平较高（p<0.05）。多因素logistic回归分析结果显示，PE患者胎盘TOS水平（OR = 1.212，95%CI = 1.068 - 1.375）是影响PE的独立危险因素。
本研究发现氧化应激是PE发生发展的独立危险因素，并缩短胎盘和脐血中的TL。未来关于端粒稳态的研究可能为PE的治疗提供新的视角。
