Wollin A
Clin Invest Med. 1987 May;10(3):136-9.
The role of the oxyntic cells in the inactivation and elimination of histamine was studied in dispersed and isolated mucosal cells. Gastric mucosal cells from rabbits were incubated with 14C-labelled histamine (1 and 3.2 X 10(-6) M) at 37 degrees C. Histamine and the histamine metabolites were extracted, separated on thin layer chromatography, and quantitated. Both the intracellular and extracellular compartments were examined. Gastric mucosal cells were found to internalize histamine and methylate it to Nt-methylhistamine, a biologically inactive metabolite. This process was Na+-dependent and occurred primarily in the oxyntic cells. Ethanol in concentrations between 1 to 4% decreased histamine methylation. The histamine content associated with the intracellular compartment was unaffected by ethanol. Pentagastrin (10(-10) to 10(-6) M) had no significant effect on histamine methylation. The results substantiate that oxyntic cells take up and inactivate histamine, and thus are capable of modifying extracellular histamine concentration. Ethanol inhibits histamine methylation, potentially maintaining or increasing extracellular histamine, while pentagastrin appears to have no significant effect.
在分散和分离的黏膜细胞中研究了壁细胞在组胺失活和消除中的作用。将来自兔子的胃黏膜细胞与14C标记的组胺（1和3.2×10(-6)M）在37℃下孵育。提取组胺及其代谢产物，在薄层色谱上分离并定量。对细胞内和细胞外部分均进行了检测。发现胃黏膜细胞摄取组胺并将其甲基化为Nτ-甲基组胺，这是一种生物活性代谢产物。该过程依赖于Na+，主要发生在壁细胞中。浓度为1%至4%的乙醇可降低组胺甲基化。与细胞内部分相关的组胺含量不受乙醇影响。五肽胃泌素（10(-10)至10(-6)M）对组胺甲基化无显著影响。结果证实壁细胞摄取并使组胺失活，因此能够改变细胞外组胺浓度。乙醇抑制组胺甲基化，可能维持或增加细胞外组胺水平，而五肽胃泌素似乎无显著影响。
