Jin Ming, Chen Huiting, Na Jigen, An Hang, Li Zhiwen, Li Nan
Institute of Reproductive and Child Health, Peking University/ Key Laboratory of Reproductive Health, National Health Commission of the People's Republic of China, Beijing 100191, PR China; Department of Epidemiology and Biostatistics, School of Public Health, Peking University Health Science Center, PR China.
Institute of Reproductive and Child Health, Peking University/ Key Laboratory of Reproductive Health, National Health Commission of the People's Republic of China, Beijing 100191, PR China; Department of Epidemiology and Biostatistics, School of Public Health, Peking University Health Science Center, PR China.
Environ Int. 2022 Dec;170:107569. doi: 10.1016/j.envint.2022.107569. Epub 2022 Oct 6.
Active smoking has been a risk factor for insomnia, however, little is known about the effect of passive smoking on insomnia. Polycyclic aromatic hydrocarbons (PAHs) are potential contents in tobacco smoke. We aimed to investigate the association between passive smoking exposure and insomnia, plus the modification effects of single nucleotide polymorphisms (SNPs) related to PAHs metabolism. A cross-sectional study with 392 nonsmoking women was conducted in a rural area of Shanxi Province, China. A structured questionnaire was used to collect the information on passive smoking exposure, insomnia and other health-related factors via face-to-face interviews. We simultaneously collected participants' hair samples to measure the concentrations of 26 PAHs. Whole blood samples were collected to test 9 SNPs related to PAHs metabolism. Logistic regression analysis was conducted to evaluate the association between passive smoking and insomnia, as well as the relation between passive smoking and PAHs concentrations. We further examined the potential interaction effects between passive smoking and SNPs on insomnia. 118 (30.1 %) were exposed to passive smoking and 128 (32.7 %) suffered from insomnia. Passive smoking nearly doubled the probability of insomnia with adjusted odds ratio and 95 % confidence interval of (1.99: 1.16, 3.39). Dose-response association between passive smoking and insomnia was observed by passive smoking frequency (P trend < 0.001). Among the 7 PAHs measured with detection rates > 70 %, no specific PAH was detected to be associated with passive smoking exposure after Bonferroni correction. The mutant genotypes of CYP1B1 (rs1056836), AHR (rs2066853), and AHRR (rs2292596) were detected to have synergetic effects with passive smoking on insomnia after multivariate adjustment. We found a positive association between passive smoking status and insomnia among nonsmoking women. This association could be modified by SNPs related to PAHs metabolism. PAHs might not be the environmentally potential mechanism involved in the insomnia caused by tobacco smoke exposure.
主动吸烟一直是失眠的一个风险因素，然而，关于被动吸烟对失眠的影响却知之甚少。多环芳烃（PAHs）是烟草烟雾中的潜在成分。我们旨在研究被动吸烟暴露与失眠之间的关联，以及与PAHs代谢相关的单核苷酸多态性（SNPs）的修饰作用。在中国山西省的一个农村地区，对392名不吸烟女性进行了一项横断面研究。通过面对面访谈，使用结构化问卷收集有关被动吸烟暴露、失眠及其他健康相关因素的信息。我们同时采集了参与者的头发样本以测量26种PAHs的浓度。采集全血样本以检测9种与PAHs代谢相关的SNPs。进行逻辑回归分析以评估被动吸烟与失眠之间的关联，以及被动吸烟与PAHs浓度之间的关系。我们进一步研究了被动吸烟与SNPs对失眠的潜在交互作用。118名（30.1%）暴露于被动吸烟，128名（32.7%）患有失眠。被动吸烟使失眠的概率几乎增加了一倍，调整后的优势比和95%置信区间为（1.99: 1.16, 3.39）。通过被动吸烟频率观察到被动吸烟与失眠之间存在剂量反应关联（P趋势<0.001）。在检测率>70%的7种PAHs中，经Bonferroni校正后，未检测到特定的PAH与被动吸烟暴露相关。在多变量调整后，检测到细胞色素P450 1B1（CYP1B1，rs1056836）、芳烃受体（AHR，rs2066853）和芳烃受体阻遏蛋白（AHRR，rs2292596）的突变基因型与被动吸烟对失眠有协同作用。我们发现不吸烟女性的被动吸烟状况与失眠之间存在正相关。这种关联可能会被与PAHs代谢相关的SNPs所修饰。PAHs可能不是烟草烟雾暴露所致失眠的环境潜在机制。
