Spencer T, Butler L J
Cytobios. 1987;50(202-203):145-50.
Five different fluorochromes were tested, in a revised fluorescence-plus-Giemsa technique, for their ability to give good quality permanent preparations. Hoechst 33342 was found to give the best results. Slides were stained with Hoechst 33342, followed by exposure to long wavelength UV light with a simultaneous incubation in a hot alkaline solution, and then were Giemsa stained. This technique gives good quality preparations, in approximately 1 h, of sister chromatid exchanges or DNA replication patterns.
在改良的荧光加吉姆萨技术中，对五种不同的荧光染料进行了测试，以评估它们制作高质量永久标本的能力。结果发现，Hoechst 33342的效果最佳。玻片先用Hoechst 33342染色，然后暴露于长波长紫外线下，同时在热碱性溶液中孵育，接着进行吉姆萨染色。该技术能在约1小时内制作出高质量的姐妹染色单体交换或DNA复制模式的标本。
