. 的单对和小群体杂交
Single-Pair and Small-Group Crosses of .
机构信息
W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA.
W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
出版信息
Cold Spring Harb Protoc. 2023 Mar 1;2023(3):108018-pdb.prot. doi: 10.1101/pdb.prot108018.
is an emerging model insect species used in genetics studies because of its ease of laboratory rearing, desiccation-resistant eggs, expanding genetic toolkit, and high-quality reference genome. Here, we describe procedures to isolate and sex virgin female and male mosquitoes and establish successful mating crosses. We also detail how to blood feed mosquitoes from these crosses, isolate individual or small groups of females for egg laying, condition these eggs for storage and hatching, and verify female mating status.
是一种新兴的模式昆虫物种，用于遗传学研究，因为它易于在实验室中饲养、具有抗干燥的卵、不断扩大的遗传工具包和高质量的参考基因组。在这里，我们描述了分离和鉴定处女雌性和雄性蚊子以及建立成功交配的程序。我们还详细介绍了如何从这些交配中给蚊子吸血，分离产卵的单个或小群雌性，对这些卵进行保存和孵化，并验证雌性的交配状态。
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