Heinicke E A, Davis H L
Exp Neurol. 1987 Sep;97(3):454-64. doi: 10.1016/0014-4886(87)90106-3.
Right extensor digitorum longus muscles of rats were denervated. After 7 days the soluble proteins were extracted from denervated and contralateral control muscles and fractionated into 32 bands by electrophoresis on polyacrylamide gels. The distribution of protein among the bands was calculated for each muscle. In denervated muscles the proportion of the total protein was increased in 11 bands and decreased in 6, relative to control muscles. Daily injection of denervated muscles with nerve extract, previously shown to offset postdenervation loss of muscle protein and shrinkage of muscle fibers, prevented the increased level of one protein and exaggerated the change in two others. Two bands, not affected by denervation, were decreased by the extract.
将大鼠右侧趾长伸肌去神经支配。7天后，从去神经支配的肌肉和对侧对照肌肉中提取可溶性蛋白质，并通过聚丙烯酰胺凝胶电泳将其分离成32条带。计算每条肌肉中各条带之间蛋白质的分布情况。与对照肌肉相比，去神经支配的肌肉中，11条带的总蛋白比例增加，6条带的总蛋白比例降低。先前的研究表明，每日向去神经支配的肌肉注射神经提取物可抵消去神经支配后肌肉蛋白的损失和肌纤维的萎缩，此次实验中该提取物阻止了一种蛋白质水平的升高，并使另外两种蛋白质的变化更加明显。有两条带不受去神经支配的影响，但却因提取物而减少。
