Christ B, Jungermann K
FEBS Lett. 1987 Sep 14;221(2):375-80. doi: 10.1016/0014-5793(87)80959-6.
[14C]Glucose release either from endogenous 14C-prelabelled glycogen or from added 14C-labelled glucose 6-phosphate was measured in filipin-treated, permeabilized hepatocytes in 48 h culture. [14C]Glucose output from prelabelled glycogen was not altered by the addition of 5 mM glucose 6-phosphate to the incubation medium. Conversely, [14C]glucose release from 5 mM labelled glucose 6-phosphate was not influenced by different glycogen concentrations in the cells. Moreover, in the permeabilized cells the anion transport inhibitor DIDS (4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid) inhibited only the liberation of [14C]glucose from labelled glucose 6-phosphate but not from glycogen. It is therefore concluded that there exist at least 2 separate, mutually non-accessible glucose 6-phosphate pools in cultured rat hepatocytes, one linked to glycogenolysis and the other to gluconeogenesis.
在培养48小时的经菲律宾菌素处理的通透化肝细胞中，测定了[14C]葡萄糖从内源性14C预标记糖原或添加的14C标记葡萄糖6-磷酸的释放情况。向孵育培养基中添加5 mM葡萄糖6-磷酸不会改变预标记糖原的[14C]葡萄糖输出。相反，细胞中不同的糖原浓度不会影响5 mM标记葡萄糖6-磷酸的[14C]葡萄糖释放。此外，在通透化细胞中，阴离子转运抑制剂DIDS（4,4'-二异硫氰酸根合芪-2,2'-二磺酸）仅抑制标记葡萄糖6-磷酸中[14C]葡萄糖的释放，而不抑制糖原中[14C]葡萄糖的释放。因此得出结论，在培养的大鼠肝细胞中至少存在2个相互独立、互不干扰的葡萄糖6-磷酸池，一个与糖原分解有关，另一个与糖异生有关。
