Curci A, Bevilacqua A, Mangia F
Dev Biol. 1987 Sep;123(1):154-60. doi: 10.1016/0012-1606(87)90437-4.
The response to heat (hs response) of preovulatory mouse oocytes was compared with that of mouse granulosa cells and characterized in regard to in vitro resumption of meiosis, amino acid incorporation into total protein, and qualitative analysis of protein synthesized before and after the shock. Granulosa cells displayed a hs response typical of other mammalian systems. When incubated at 43 degrees C for 20-40 min, these cells maintained a normal level of amino acid incorporation into total protein, responded to stress by new synthesis of 33- and 68-kDa heat-shock proteins (hsps), and enhanced synthesis of 70-kDa heat-shock cognate protein (hsc70) and of 89- and 110-kDa hsps. In contrast to granulosa cells, preovulatory mouse oocytes were very sensitive to hyperthermia. Incubation at 43 degrees C for 20-40 min strongly inhibited oocyte resumption of meiosis and protein synthesis and did not induce a new or enhanced synthesis of hsps. Unstressed preovulatory mouse oocytes constitutively synthesized 70- and 89-kDa polypeptides resembling hsc70 and hsp89 of granulosa cells.
将排卵前小鼠卵母细胞的热反应（hs反应）与小鼠颗粒细胞的热反应进行比较，并从体外减数分裂恢复、氨基酸掺入总蛋白以及休克前后合成蛋白的定性分析方面对其进行表征。颗粒细胞表现出其他哺乳动物系统典型的hs反应。当在43℃孵育20 - 40分钟时，这些细胞维持氨基酸掺入总蛋白的正常水平，通过新合成33 kDa和68 kDa热休克蛋白（hsps）对压力作出反应，并增强70 kDa热休克同源蛋白（hsc70）以及89 kDa和110 kDa hsps的合成。与颗粒细胞相反，排卵前小鼠卵母细胞对热疗非常敏感。在43℃孵育20 - 40分钟会强烈抑制卵母细胞的减数分裂恢复和蛋白合成，并且不会诱导hsps的新合成或增强合成。未受应激的排卵前小鼠卵母细胞组成性合成类似于颗粒细胞hsc70和hsp89的70 kDa和89 kDa多肽。
