College of Science and Research Center for the Natural and Applied Sciences, University of Santo Tomas, Manila 1015, Philippines.
Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8675, Japan.
Nutrients. 2022 Sep 24;14(19):3962. doi: 10.3390/nu14193962.
Accumulation of amyloid- (Aβ) plaques leading to oxidative stress, mitochondrial damage, and cell death is one of the most accepted pathological hallmarks of Alzheimer's disease (AD). , commonly recognized as fragrant screw pine due to its characteristic smell, is widely distributed in Southeast Asia and is consumed as a food flavor. In search for potential anti-AD agents from terrestrial sources, was explored for its in vitro anti-amyloidogenic and neuroprotective effects. Thioflavin T (ThT) assay and the high-throughput screening multimer detection system (MDS-HTS) assay were used to evaluate the extracts' potential to inhibit Aβ aggregations and oligomerizations, respectively. The crude alcoholic extract (CAE, 50 μg/mL) and crude base extract (CBE, 50 μg/mL) obstructed the Aβ aggregation. Interestingly, results revealed that only CBE inhibited the Aβ nucleation at 100 μg/mL. Both CAE and CBE also restored the cell viability, reduced the level of reactive oxygen species, and reversed the mitochondrial dysfunctions at 10 and 20 μg/mL extract concentrations in Aβ-insulted SY-SY5Y cells. In addition, the unprecedented isolation of nicotinamide from CBE is a remarkable discovery as one of its potential bioactive constituents against AD. Hence, our results provided new insights into the promising potential of extracts against AD and further exploration of other prospective bioactive constituents.
淀粉样蛋白(Aβ)斑块的积累导致氧化应激、线粒体损伤和细胞死亡,这是阿尔茨海默病(AD)最被认可的病理特征之一。飘香藤,因其特有的气味而通常被认为是香藤,广泛分布于东南亚,被用作食品香料。为了从陆地资源中寻找潜在的抗 AD 药物,对飘香藤进行了体外抗淀粉样蛋白形成和神经保护作用的研究。使用硫黄素 T(ThT) assay 和高通量筛选多聚体检测系统(MDS-HTS)assay 分别评估提取物抑制 Aβ聚集和寡聚的潜力。粗醇提取物(CAE,50μg/mL)和粗碱提取物(CBE,50μg/mL)阻止了 Aβ聚集。有趣的是,结果表明只有 CBE 在 100μg/mL 时抑制了 Aβ成核。CAE 和 CBE 还在 Aβ 损伤的 SY-SY5Y 细胞中,在 10 和 20μg/mL 提取物浓度下,恢复了细胞活力,降低了活性氧水平,并逆转了线粒体功能障碍。此外,从 CBE 中分离出烟酰胺是一个值得注意的发现,因为它是一种有希望的抗 AD 生物活性成分。因此,我们的结果为飘香藤提取物具有抗 AD 的潜力提供了新的见解,并进一步探索了其他有前途的生物活性成分。