Wilairat Monwilun, Rotpenpian Nattapon
Department of Oral Biology and Occlusion, Faculty of Dentistry, Prince of Songkla University, Songkhla, Thailand.
J Oral Biol Craniofac Res. 2025 Sep-Oct;15(5):919-924. doi: 10.1016/j.jobcr.2025.06.015. Epub 2025 Jun 21.
This study aimed to evaluate the antimicrobial activity, cytotoxicity and cell migration of ethanolic extract of (PAE).
The antimicrobial activity of PAE was tested against ) using the microdilution assay and determing minimum inhibitory and bactericidal concentration (MIC and MBC). Additionally, biofilm inhibition was assessed by crystal violet assay at different concentrations of PAE. Moreover, a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and scratch assays were performed to assess cell viability and migration of human gingival fibroblasts (HGFs).
The MIC and MBC values of PAE against were determined to be 16 mg/mL and 32 mg/mL, respectively. Significant biofilm inhibition was observed at concentrations of 8 mg/mL and 4 mg/mL. The MTT assay demonstrated that 4 mg/mL of PAE was non-toxic to HGFs, whereas higher concentrations exhibited cytotoxic effects. Furthermore, PAE at 4, 2, 1, and 0.5 mg/mL promoted wound healing in an in vitro model.
PAE exhibited cytotoxic effects on HGFs at concentrations above 4 mg/mL. However, lower concentrations promoted cell migration and demonstrated potential antimicrobial activity against . These findings suggest that PAE may have applications in oral health for antimicrobial and wound-healing purposes.
本研究旨在评估[植物名称]乙醇提取物（PAE）的抗菌活性、细胞毒性和细胞迁移能力。
采用微量稀释法测定PAE对[细菌名称]的抗菌活性，并确定最低抑菌浓度和最低杀菌浓度（MIC和MBC）。此外，通过结晶紫测定法评估不同浓度PAE对生物膜的抑制作用。此外，进行3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）和划痕试验，以评估人牙龈成纤维细胞（HGFs）的细胞活力和迁移能力。
PAE对[细菌名称]的MIC和MBC值分别确定为16mg/mL和32mg/mL。在8mg/mL和4mg/mL浓度下观察到显著的生物膜抑制作用。MTT试验表明，4mg/mL的PAE对HGFs无毒，而较高浓度则表现出细胞毒性作用。此外，4、2、1和0.5mg/mL的PAE在体外模型中促进了伤口愈合。
PAE在浓度高于4mg/mL时对HGFs表现出细胞毒性作用。然而，较低浓度促进了细胞迁移，并显示出对[细菌名称]的潜在抗菌活性。这些发现表明，PAE可能在口腔健康中用于抗菌和伤口愈合目的。
