Janković B D, Marić D
Immunol Lett. 1987 Jun;15(2):153-60. doi: 10.1016/0165-2478(87)90047-2.
Wistar rats were sensitized for anaphylactic shock with ovalbumin and treated intraperitoneally with 10 injections of methionine-enkephalin and leucine-enkephalin (4 mg/kg of body weight). Both pentapeptides suppressed the development of systemic anaphylaxis after intravenous injection of a shocking dose of ovalbumin. Methionine-enkephalin completely protected the animals from fatal shock. The level of circulating IgE and precipitating anti-ovalbumin antibodies decreased in rats given 10 injections of enkephalins. Mast cell degranulation was significantly inhibited in these animals as observed at autopsy. A single intraperitoneal injection of 4 mg/kg body weight of methionine-enkephalin given 30 min before the challenge with shock-inducing dose of antigen was also effective, although to a lesser extent, in protecting animals from anaphylactic shock. One injection of leucine-enkephalin did not exhibit significant antishock activity. The results suggest that enkephalins, and methionine-enkephalin in particular, are potent modulators of the complex biochemical processes underlying the anaphylactic shock in the rat.
用卵清蛋白使Wistar大鼠致敏以引发过敏性休克，并对其进行腹腔注射，给予10次甲硫氨酸脑啡肽和亮氨酸脑啡肽（4毫克/千克体重）。在静脉注射致死剂量的卵清蛋白后，这两种五肽均抑制了全身性过敏反应的发展。甲硫氨酸脑啡肽完全保护动物免于致命休克。给予10次脑啡肽注射的大鼠体内循环IgE水平和沉淀抗卵清蛋白抗体水平降低。尸检时观察到，这些动物的肥大细胞脱颗粒受到显著抑制。在给予诱导休克剂量抗原攻击前30分钟，腹腔注射一次4毫克/千克体重的甲硫氨酸脑啡肽，尽管保护动物免于过敏性休克的效果较小，但同样有效。单次注射亮氨酸脑啡肽未表现出显著的抗休克活性。结果表明，脑啡肽，尤其是甲硫氨酸脑啡肽，是大鼠过敏性休克潜在复杂生化过程的有效调节剂。
