Brady M S, Katz S E
J Assoc Off Anal Chem. 1987 Jul-Aug;70(4):641-6.
A protocol for microbial assays of antibiotics, using the plate diffusion system, is presented. The system is based on the concept that a complete standard curve and assay unknowns can be placed on an assay plate and that 2 plates can be a complete assay with an accuracy and precision essentially equivalent to the official AOAC diffusion procedure. Four antibiotics, bacitracin, chlortetracycline, oxytetracycline, and streptomycin, were used in the design and comparison studies with the AOAC protocol. The coefficients of variation (CVs) for the AOAC design, using 10 replicates, ranged from 1.4 to 10.3% with a mean of 4.5%. The CVs for the single-plate option of the simplified design ranged from 4.3 to 9.6% with a mean of 6.6%; the CVs for the 2-plate option ranged from 2.5 to 6.8% with a mean of 4.3%; the CVs for the 3-plate option ranged from 1.2 to 5.0% with a mean of 3.0%.
本文介绍了一种使用平板扩散系统进行抗生素微生物检测的方案。该系统基于这样一种理念：完整的标准曲线和检测未知物可以放置在一个检测平板上，并且两个平板就可以构成一次完整的检测，其准确度和精密度基本等同于美国官方分析化学家协会（AOAC）的扩散程序。在与AOAC方案的设计和比较研究中使用了四种抗生素，即杆菌肽、金霉素、土霉素和链霉素。AOAC设计采用10次重复，变异系数（CV）范围为1.4%至10.3%，平均值为4.5%。简化设计的单平板方案的CV范围为4.3%至9.6%，平均值为6.6%；双平板方案的CV范围为2.5%至6.8%，平均值为4.3%；三平板方案的CV范围为1.2%至5.0%，平均值为3.0%。
