多毛纲沙蚕肌浆钙结合蛋白的结晶及初步X射线研究
Crystallization and preliminary x-ray investigation of sarcoplasmic calcium-binding protein from Nereis diversicolor.
作者信息
Babu Y S, Cox J A, Cook W J
出版信息
J Biol Chem. 1987 Aug 25;262(24):11884-5.
PMID:3624239
Abstract
Crystals of sarcoplasmic calcium-binding proteins from Nereis diversicolor have been grown from solutions of ammonium sulfate. The crystals are monoclinic, space group P2(1); the axes are a = 43.65 (1), b = 56.05 (1), c = 65.77 (1) A, and beta = 92.58 (2) degrees. The crystals are quite stable to x-rays and diffract beyond 2.5 A resolution. The asymmetric unit contains two protein molecules.
摘要
来自多毛纲沙蚕的肌浆钙结合蛋白晶体已从硫酸铵溶液中生长出来。这些晶体为单斜晶系，空间群为P2(1)；轴长分别为a = 43.65 (1)、b = 56.05 (1)、c = 65.77 (1) Å，β = 92.58 (2) 度。这些晶体对X射线相当稳定，衍射分辨率超过2.5 Å。不对称单元包含两个蛋白质分子。
相似文献
1
Crystallization and preliminary x-ray investigation of sarcoplasmic calcium-binding protein from Nereis diversicolor.多毛纲沙蚕肌浆钙结合蛋白的结晶及初步X射线研究
J Biol Chem. 1987 Aug 25;262(24):11884-5.
2
Crystallization and preliminary X-ray investigation of a sarcoplasmic calcium-binding protein from amphioxus.
J Mol Biol. 1991 Oct 20;221(4):1071-3. doi: 10.1016/0022-2836(91)90917-u.
3
Calmodulin, S-100, and crayfish sarcoplasmic calcium-binding protein crystals suitable for X-ray diffraction studies.
J Biol Chem. 1980 Sep 10;255(17):8154-6.
4
Structure of a sarcoplasmic calcium-binding protein from Nereis diversicolor refined at 2.0 A resolution.多毛纲杂色沙蚕肌浆钙结合蛋白在2.0埃分辨率下的结构精制
J Mol Biol. 1992 Mar 20;224(2):413-26. doi: 10.1016/0022-2836(92)91004-9.
5
Crystallization and preliminary X-ray investigation of calmodulin.
J Biol Chem. 1980 Sep 10;255(17):8152-3.
6
Amino acid sequence of a sarcoplasmic calcium-binding protein from the sandworm Nereis diversicolor.多毛纲沙蚕肌质钙结合蛋白的氨基酸序列
J Biol Chem. 1988 Oct 25;263(30):15378-85.
7
Crystallization and preliminary characterization of CheY, a chemotaxis control protein from Escherichia coli.
J Biol Chem. 1986 Apr 5;261(10):4723-5.
8
Crystallization and preliminary X-ray diffraction studies of aspartic proteinase from Irpex lacteus.
J Mol Biol. 1992 Aug 20;226(4):1291-3. doi: 10.1016/0022-2836(92)91070-6.
9
Purification and crystallization of the calcium binding protein of sarcoplasmic reticulum from skeletal muscle.从骨骼肌中分离纯化肌浆网钙结合蛋白并进行结晶
Proc Natl Acad Sci U S A. 1985 Jun;82(12):4036-40. doi: 10.1073/pnas.82.12.4036.
10
Crystallization and preliminary X-ray investigation of a ubiquitin carrier protein (E2) from Arabidopsis thaliana.来自拟南芥的泛素载体蛋白（E2）的结晶及初步X射线研究。
J Mol Biol. 1992 Feb 20;223(4):1183-6. doi: 10.1016/0022-2836(92)90270-t.
引用本文的文献
1
Cloning, characterization, and heterologous expression of the Saccharopolyspora erythraea (Streptomyces erythraeus) gene encoding an EF-hand calcium-binding protein.编码一种EF-手型钙结合蛋白的糖多孢红霉菌（红色链霉菌）基因的克隆、表征及异源表达
J Bacteriol. 1989 Oct;171(10):5614-9. doi: 10.1128/jb.171.10.5614-5619.1989.
Zotero 插件