School of Ecology and Environment, Zhengzhou University, Zhengzhou, Henan, 450001, PR China.
College of Public Health, Zhengzhou University, Zhengzhou, Henan, 450001, PR China; Research Center for Eco-friendly Wastewater Purifying Engineering Technology of Henan Province, Henan Junhe Environmental Protection Technology CO.LTD, Zhengzhou, Henan, 450001, PR China.
Chemosphere. 2023 Jan;310:136854. doi: 10.1016/j.chemosphere.2022.136854. Epub 2022 Oct 13.
High ammonia concentration hinders the efficient treatment of antibiotic production wastewater (APW). Developing effective ammonia oxidation wastewater treatment strategies is an ideal approach for facilitating APW treatment. Compared with traditional nitrification strategies, the partial nitrification process is more eco-friendly, less energy-intensive, and less excess sludge. The primary limiting factor of the partial nitrification process is increasing ammonia-oxidizing bacteria (AOB) while decreasing nitrite-oxidizing bacteria (NOB). In this study, an efficient AOB microbiota (named AF2) was obtained via enrichment of an aerobic activated sludge (AS0) collected from a pharmaceutical wastewater treatment plant. After a 52-day enrichment of AS0 in 250 mL flasks, the microbiota AE1 with 69.18% Nitrosomonas microorganisms was obtained. Subsequent scaled-up cultivation in a 10 L fermenter led to the AF2 microbiota with 59.22% Nitrosomonas. Low concentration of free ammonia (FA, < 42.01 mg L) had a negligible effect on the activity of AF2, and the nitrite-nitrogen accumulation rate (NAR) of AF2 was 98% when FA concentration was 42.01 mg L. The specific ammonia oxidation rates (SAORs) at 30 °C and 15 °C were 3.64 kg NH-N·kg MLVSS·d and 1.43 kg NH-N·kg MLVSS·d (MLVSS: mixed liquor volatile suspended solids). The SAOR was 0.52 kg NH-N·kg MLVSS·d when the NaCl concentration was increased from 0 to 20 g L, showing that AF2 functioning was stable in a high-level salt environment. The ammonia oxidation performance of AF2 was verified by treating abamectin and lincomycin production wastewater. The NARs of AF2 used for abamectin and lincomycin production wastewater treatment were >90% and the SAORs were 2.39 kg NH-N·kg MLVSS·d and 0.54 kg NH-N·kg MLVSS·d, respectively, which was higher than the traditional biological denitrification process. In summary, AF2 was effective for APW treatment via enhanced ammonia removal efficiency, demonstrating great potential for future industrial wastewater treatment.
高浓度氨会阻碍抗生素生产废水（APW）的有效处理。开发有效的氨氧化废水处理策略是促进 APW 处理的理想方法。与传统硝化策略相比，部分硝化工艺更环保、能源消耗更少、剩余污泥更少。部分硝化工艺的主要限制因素是增加氨氧化细菌（AOB）的同时减少亚硝酸盐氧化细菌（NOB）。在这项研究中，通过富集从制药废水处理厂收集的好氧活性污泥（AS0），获得了一种高效的 AOB 微生物群（命名为 AF2）。在 250 毫升烧瓶中对 AS0 进行 52 天的富集后，获得了微生物群 AE1，其中 69.18%的硝化单胞菌。随后在 10 升发酵罐中进行扩大培养，得到了 59.22%的硝化单胞菌。低浓度游离氨（FA，<42.01mg/L）对 AF2 的活性几乎没有影响，当 FA 浓度为 42.01mg/L 时，AF2 的亚硝酸盐氮积累率（NAR）为 98%。在 30°C 和 15°C 时，AF2 的比氨氧化速率（SAOR）分别为 3.64kg NH-N·kg MLVSS·d 和 1.43kg NH-N·kg MLVSS·d（MLVSS：混合液挥发性悬浮固体）。当 NaCl 浓度从 0 增加到 20g/L 时，SAOR 为 0.52kg NH-N·kg MLVSS·d，表明 AF2 在高盐环境中运行稳定。通过处理阿维菌素和林可霉素生产废水验证了 AF2 的氨氧化性能。AF2 用于阿维菌素和林可霉素生产废水处理的 NAR 均>90%，SAOR 分别为 2.39kg NH-N·kg MLVSS·d 和 0.54kg NH-N·kg MLVSS·d，高于传统生物脱氮工艺。综上所述，AF2 通过提高氨去除效率，有效地处理了 APW，在未来的工业废水处理中具有很大的潜力。
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