利用表面增强拉曼光谱揭示G-四链体与配体之间的特定相互作用。

Revealing the specific interactions between G-quadruplexes and ligands by surface-enhanced Raman spectroscopy.

作者信息

Bao Ying, Zhang Xiaonong, Xiang Xiaoxuan, Zhang Yujing, Guo Xinhua

机构信息

State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012, PR China.

Key Laboratory of Polymer Ecomaterials, Jilin Biomedical Polymers Engineering Laboratory, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.

出版信息

Int J Biol Macromol. 2022 Dec 1;222(Pt B):2948-2956. doi: 10.1016/j.ijbiomac.2022.10.070. Epub 2022 Oct 12.

DOI:10.1016/j.ijbiomac.2022.10.070

PMID:36243165

Abstract

G-quadruplexes (G4s) regulate a variety of physiological functions related to diseases and life elongation. Therefore, G4 binding ligands, such as potential drugs in gene therapy or molecular probes for biosensing and bioimaging, are receiving extensive attention. However, identifying the binding modes and interaction details between G4s and their ligands is very challenging. Recently, we demonstrated that surface-enhanced Raman scattering (SERS) could quickly provide structural details of G4s. Herein, three G4 binding ligands that interact with the separated G4 in different ways are selected as models to evaluate the feasibility of SERS analysis in studying G4-ligand interactions. As a result, adequate SERS information indicating the specific interactions between the G4s and the ligand is obtained via using Ag IANPs as substrates. The results demonstrate that SERS is a powerful tool for revealing comprehensive and specific ligand-DNA interactions with advantages such as speed, simplicity, trace sample amount requirement, and compatibility with aqueous samples.

摘要

G-四链体（G4s）调节与疾病和寿命延长相关的多种生理功能。因此，G4结合配体，如基因治疗中的潜在药物或用于生物传感和生物成像的分子探针，正受到广泛关注。然而，确定G4s与其配体之间的结合模式和相互作用细节极具挑战性。最近，我们证明表面增强拉曼散射（SERS）可以快速提供G4s的结构细节。在此，选择三种以不同方式与分离的G4相互作用的G4结合配体作为模型，以评估SERS分析在研究G4-配体相互作用中的可行性。结果，通过使用Ag IANPs作为底物，获得了表明G4s与配体之间特定相互作用的足够SERS信息。结果表明，SERS是揭示全面且特定的配体-DNA相互作用的有力工具，具有速度快、操作简单、所需样品量少以及与水性样品兼容等优点。