Shapiro H S
J Chromatogr. 1987 May 22;394(2):315-22. doi: 10.1016/s0021-9673(01)94183-4.
The population of pyrimidine deoxyribo-oligonucleotides obtained from DNA may be separated, first, into fractions comprising identical lengths (isotichs) and, subsequently, into the individual components of an isostich fraction varying in the cytidylic and thymidylic acid composition. At a third level of analysis the proportion of components exhibiting the same composition but different sequence may be determined. Selective removal of cytosine and the subsequent analysis for characteristic thymidine derivatives released during an acid-catalyzed beta-elimination reaction allowed estimation of the relative proportion of sequence isomers for pyrimidine isostichs of length two to five.
从DNA中获得的嘧啶脱氧核糖寡核苷酸群体，首先可分离成具有相同长度的片段（同聚物），随后再分离成同聚物片段中胞苷酸和胸苷酸组成不同的各个组分。在第三个分析层面，可以确定具有相同组成但序列不同的组分比例。通过选择性去除胞嘧啶，并对酸催化的β-消除反应过程中释放的特征性胸苷衍生物进行后续分析，能够估算长度为二至五个核苷酸的嘧啶同聚物序列异构体的相对比例。
