采用毛细管气相色谱法测定地尔硫䓬和去乙酰地尔硫䓬。
Assay of diltiazem and deacetyldiltiazem by capillary gas chromatography.
作者信息
Grech-Belanger O, Leboeuf E, Langlois S
出版信息
J Chromatogr. 1987 Jun 5;417(1):89-98. doi: 10.1016/0378-4347(87)80094-4.
A highly sensitive gas chromatographic method for the analysis of diltiazem and deacetyldiltiazem in plasma or serum is reported. After silylation with bis (trimethylsilyl) trifluoroacetamide, separation was obtained on a cross-linked fused-silica column and detection was by electron-capture. The minimum measurable concentrations were 3 and 1 ng/ml for diltiazem and deacetyldiltiazem, respectively. Intra- and inter-day coefficients of variation were less or equal to 6.0 and 8.0%, respectively, for both compounds. The method was used to study the kinetics of a single oral dose of 60 mg of diltiazem hydrochloride in a patient with renal failure.
报道了一种用于分析血浆或血清中地尔硫䓬和去乙酰地尔硫䓬的高灵敏度气相色谱法。用双（三甲基硅基）三氟乙酰胺进行硅烷化处理后，在交联熔融石英柱上进行分离，并通过电子捕获进行检测。地尔硫䓬和去乙酰地尔硫䓬的最低可测浓度分别为3和1 ng/ml。两种化合物的日内和日间变异系数分别小于或等于6.0%和8.0%。该方法用于研究一名肾衰竭患者单次口服60 mg盐酸地尔硫䓬的动力学。
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