George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu-Mures, 38 Gheorghe Marinescu Str., 540139, Targu Mures, Romania.
Technical University of Cluj-Napoca, 28 Memorandumului Str., 400114, Cluj-Napoca, Romania.
Biochem Biophys Res Commun. 2022 Dec 17;634:168-174. doi: 10.1016/j.bbrc.2022.09.114. Epub 2022 Oct 7.
Classical histological methods such as hematoxylin-eosin staining, have been, and in some areas still are, an important benchmark for the evaluation of biological tissues. However, the current method of assessment is primarily a qualitative assessment of the tissue under investigation. The aim of this paper is to contribute to the improvement of classical histological methods, by applying physical techniques that allow objective, quantitative data to be added to qualitative assessments, especially in areas where conflicting results are available. To this end, the effect of hypolipidemic medication on the callus formation process of normal bone and pathological osteoporotic bone was investigated. The study allowed us to associate UV-VIS spectroscopy wave number with specific hematoxylin-eosin staining of different types of bone tissue structures, the evolving structures in the callus formation process. This association allowed the quantitative assessment of the callusing process in ovariectomized (associated with pathological, osteoporotic bone) and non-ovariectomized (associated with normal bone) rats, with three groups - the control group, simvastatin-treated group, and fenofibrate-treated group. The study showed that in the non-ovariectomized groups both treatments delayed callus formation. In the ovariectomized groups, simvastatin delayed and fenofibrate promoted callus formation.
经典的组织学方法，如苏木精-伊红染色，一直是，并且在某些领域仍然是，评估生物组织的重要基准。然而，目前的评估方法主要是对所研究组织的定性评估。本文的目的是通过应用物理技术来改进经典的组织学方法，这些技术可以在定性评估的基础上添加客观的定量数据，特别是在存在相互矛盾的结果的领域。为此，研究了降脂药物对正常骨和病理性骨质疏松骨的骨痂形成过程的影响。该研究使我们能够将 UV-VIS 光谱波数与不同类型骨组织结构的特定苏木精-伊红染色相关联，这些结构在骨痂形成过程中不断演变。这种关联允许对去卵巢（与病理性、骨质疏松骨相关）和非去卵巢（与正常骨相关）大鼠的骨痂形成过程进行定量评估，分为三组 - 对照组、辛伐他汀治疗组和非诺贝特治疗组。研究表明，在非去卵巢组中，两种治疗方法都延迟了骨痂形成。在去卵巢组中，辛伐他汀延迟了骨痂形成，而非诺贝特促进了骨痂形成。
