Li Chi, Wang Jing-Han, Yu Chong, Zhang Jing-Tian, Chi Zhan-You, Zhang Qian
School of Bioengineering, Dalian University of Technology, Dalian 116024, PR China.
School of Bioengineering, Dalian University of Technology, Dalian 116024, PR China; Key Laboratory of Environment Controlled Aquaculture, Dalian Ocean University, Dalian 116023, PR China.
Bioresour Technol. 2022 Nov;364:128117. doi: 10.1016/j.biortech.2022.128117. Epub 2022 Oct 13.
Using low strength wastewater for microalgae cultivation is challenged by slow growth and biomass harvesting issue in suspended systems, and growth-promoting effects of phytohormones at currently recommended dosages could neither obtain high enough biomass concentrations nor economic feasibility. This study aims to solve the issues of slow growth, biomass harvest, and phytohormone costs altogether by supplementing low dosage phytohormones in an improved capillary-driven attached cultivation device. The device displayed nutrients-condensing properties, and dosages of indole acetic acid (IAA), 6-benzylaminopurine (6-BA), and salicylic acid (SA) for highest microalgal growth were respectively 10 M, 10 M, and 10 M, being at least one order of magnitude lower than in suspended cultures. SA was most effective in growth-promoting (up to 7.0 g/m biomass density) and nutrients uptake (up to 98.6 % from the bulk environment), while IAA was most effective in antioxidative defenses. These results provided new insights in cost-effective and harvesting-convenient microalgae production.
在悬浮系统中，利用低强度废水培养微藻面临着生长缓慢和生物量收获的问题，而且目前推荐剂量的植物激素的促生长作用既无法获得足够高的生物量浓度，也缺乏经济可行性。本研究旨在通过在改进的毛细管驱动附着式培养装置中补充低剂量植物激素，一并解决生长缓慢、生物量收获和植物激素成本等问题。该装置具有营养物质浓缩特性，促进微藻生长的吲哚乙酸（IAA）、6-苄基腺嘌呤（6-BA）和水杨酸（SA）的剂量分别为10 μM、10 μM和10 μM，至少比悬浮培养中的剂量低一个数量级。SA在促进生长（生物量密度高达7.0 g/m）和营养物质吸收（从整体环境中吸收高达98.6%）方面最有效，而IAA在抗氧化防御方面最有效。这些结果为经济高效且收获方便的微藻生产提供了新的见解。
