He Zhongqi, Zhou Xu, Mei Nannan, Jin Wenbiao, Sun Jing, Yin Shiyu, Wang Qilin
State Key Laboratory of Urban Water Resource and Environment, School of Civil and Environmental Engineering, Harbin Institute of Technology (Shenzhen), Shenzhen, Guangdong 518055, China.
State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
Water Res X. 2024 Aug 29;24:100254. doi: 10.1016/j.wroa.2024.100254. eCollection 2024 Sep 1.
Microalgae biofilm has garnered significant attention from researchers in the field of sewage treatment due to its advantages such as ease of collection and stable sewage treatment capabilities. Using agricultural waste as biofilm carriers has become a hotspot in reducing costs for this method. This study first combined with loofah to form a microalgae biofilm for the study of periodic nitrogen and phosphorus removal from municipal wastewater. The biofilm could stably treat 7 batches of wastewater within one month. The removal rate of TP almost reached 100 %, while the removal rates of NH and TN both reached or exceeded 80 %. The average biomass yield over 25 days was 102.04 mg/L/day. The polysaccharide content increased from 8.61 % to 16.98 % during the cyclic cultivation. The lipid content gradually decreased from 40.91 to 26.1 %. The protein content increased from 32.93 % in the initial stage to 41.18 % and then decreased to 36.31 % in the later stage. During the mid-stage of culturing, the richness of anaerobic bacteria decreased, while the richness of aerobic and facultative bacteria increased, which was conducive to the construction of the microalgae-bacteria symbiotic system and steadily improved the effect of nitrogen and phosphorus removal. As the culturing progressed, the that emerged during the mid-stage gradually damaged the biofilm over time, leading to a decline in the effectiveness of sewage treatment in the later stages. This study offers technical support for carrier selection in microalgae biofilm methods and for the periodic removal of nitrogen and phosphorus from wastewater.
微藻生物膜因其易于收集和稳定的污水处理能力等优点，在污水处理领域受到了研究人员的广泛关注。利用农业废弃物作为生物膜载体已成为降低该方法成本的一个热点。本研究首先将丝瓜与微藻结合形成微藻生物膜，用于研究城市污水中氮和磷的周期性去除。该生物膜在一个月内可稳定处理7批次废水。TP的去除率几乎达到100%，而NH₃和TN的去除率均达到或超过80%。25天内的平均生物量产量为102.04mg/L/天。在循环培养过程中，多糖含量从8.61%增加到16.98%。脂质含量从40.91%逐渐降至26.1%。蛋白质含量从初始阶段的32.93%增加到41.18%，后期又降至36.31%。在培养中期，厌氧菌的丰富度下降，而好氧菌和兼性菌的丰富度增加，这有利于微藻-细菌共生系统的构建，并稳定提高了氮和磷的去除效果。随着培养的进行，中期出现的[此处原文缺失相关内容]随着时间的推移逐渐破坏生物膜，导致后期污水处理效果下降。本研究为微藻生物膜法中的载体选择以及废水中氮和磷的周期性去除提供了技术支持。
