Yao Min, Cai Yin, Wu Zhi-Jun, Zhou Ping, Sai Wen-Li, Wang De-Feng, Wang Li, Yao Deng-Fu
Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China.
Department of Medical Immunology, Medical School of Nantong University, Nantong 226001, Jiangsu Province, China.
World J Clin Cases. 2022 Oct 6;10(28):10017-10030. doi: 10.12998/wjcc.v10.i28.10017.
Insulin-like growth factor-1 receptor (IGF-1R) is over-expressed in hepatocellular carcinoma (HCC). However, the relationship between activation and HCC progression remains unidentified.
To investigate the effects of editing on the biological features of HCC cells.
Immunohistochemistry analyzed the expressions of IGF-1R and P-glyco protein (P-gp) in HCC tissues and their distal non-cancerous tissues (non-Ca). was edited with Crispr/Cas9 system, screened specific sgRNAs, and then transfected into HepG2 cells. CCK-8, scratch wound test detected cell proliferation, migration, invasion and transwell assays, respectively. Alterations of IGF-1R and P-gp were confirmed by Western blotting. Alterations of anti-cancer drug IC values were analyzed at the cell level.
The positive rates of IGF-1R (93.6%, = 63.947) or P-gp (88.2%, χ = 58.448) were significantly higher ( < 0.001) in the HCC group than those (36.6% in IGF-1R or 26.9% in P-gp) in the non-Ca group. They were positively correlated between high IGF-1R and P-gp expression, and they were associated with hepatitis B virus infection and vascular invasion of HCC. Abnormal expressions of circulating IGF-1R and P-gp were confirmed and associated with HCC progression. Biological feature alterations of HCC cells transfected with specific sgRNA showed IGF-1R expression down-regulation, cell proliferation inhibition, cell invasion or migration potential decreasing, and enhancing susceptibility of HepG2 cells to anti-cancer drugs.
Edited oncogenic was useful to inhibit biological behaviors of HepG2 cells.
胰岛素样生长因子-1受体（IGF-1R）在肝细胞癌（HCC）中过表达。然而，其激活与HCC进展之间的关系仍不明确。
研究编辑对HCC细胞生物学特性的影响。
免疫组织化学分析IGF-1R和P-糖蛋白（P-gp）在HCC组织及其远端非癌组织（非癌）中的表达。用Crispr/Cas9系统对其进行编辑，筛选特异性sgRNA，然后转染至HepG2细胞。分别采用CCK-8、划痕试验检测细胞增殖、迁移、侵袭，并进行Transwell试验。通过蛋白质免疫印迹法确认IGF-1R和P-gp的变化。在细胞水平分析抗癌药物IC值的变化。
HCC组中IGF-1R（93.6%，χ² = 63.947）或P-gp（88.2%，χ² = 58.448）的阳性率显著高于非癌组（IGF-1R为36.6%或P-gp为26.9%）（P < 0.001）。高IGF-1R与P-gp表达呈正相关，且与乙型肝炎病毒感染及HCC的血管侵犯有关。循环IGF-1R和P-gp的异常表达得到确认，并与HCC进展相关。转染特异性sgRNA的HCC细胞生物学特性改变表现为IGF-1R表达下调、细胞增殖受抑制、细胞侵袭或迁移能力降低，以及HepG2细胞对抗癌药物的敏感性增强。
编辑致癌基因对抑制HepG2细胞的生物学行为有效。
