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支架拓扑结构与培养条件的协同效应：一种用于髓核组织工程的方法。

The combinatory effect of scaffold topography and culture condition: an approach to nucleus pulposus tissue engineering.

作者信息

Vanawati Noviana, Barlian Anggraini, Judawisastra Hermawan, Wibowo Indra

机构信息

School of Life Sciences & Technology, Institut Teknologi Bandung, Bandung, West Java, 40132, Indonesia.

Faculty of Mechanical & Aerospace Engineering, Institut Teknologi Bandung, Bandung, West Java, 40132, Indonesia.

出版信息

Future Sci OA. 2022 Oct 3;8(7):FSO810. doi: 10.2144/fsoa-2021-0157. eCollection 2022 Aug.

DOI:10.2144/fsoa-2021-0157
PMID:36248063
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9540240/
Abstract

UNLABELLED

Scaffold topography and culture medium conditions for human wharton's jelly mesenchymal stem cells (hWJ-MSC) are critical components of the approach to nucleus pulposus (NP) tissue engineering.

AIM

To evaluate the silk fibroin (SF) scaffold topography analysis (optimal thickness and pore diameter) and to determine culture medium conditions for the growth and differentiation of hWJ-MSC.

METHOD

hWJ-MSCs were seeded into different thicknesses and pore size diameters and grown in different concentrations of glucose, platelet rich plasma (PRP) and oxygen. The cell-seeded scaffold was evaluated for cell attachment, growth and differentiation potency.

RESULTS & DISCUSSION: The results indicated that SF scaffold with a minimum thickness 3.5 mm and pore diameter of 500 μm with cells cultured under low glucose, 10% PRP and normoxia conditions induced the growth and differentiation of hWJ-MSCs, indicated by the accumulation of glycosaminoglycans content and the presence of type II collagen, as markers of NP-like cells.

摘要

未标注

人脐带华通氏胶间充质干细胞（hWJ-MSC）的支架拓扑结构和培养基条件是髓核（NP）组织工程方法的关键组成部分。

目的

评估丝素蛋白（SF）支架拓扑结构分析（最佳厚度和孔径），并确定hWJ-MSC生长和分化的培养基条件。

方法

将hWJ-MSCs接种到不同厚度和孔径的支架上，并在不同浓度的葡萄糖、富血小板血浆（PRP）和氧气条件下培养。对接种细胞的支架进行细胞附着、生长和分化潜能评估。

结果与讨论

结果表明，在低葡萄糖、10% PRP和常氧条件下培养细胞时，最小厚度为3.5 mm、孔径为500 μm的SF支架可诱导hWJ-MSCs的生长和分化，这通过糖胺聚糖含量的积累和II型胶原蛋白的存在得以体现，这些是NP样细胞的标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cb8/9540240/4d92cf1b80bb/fsoa-08-810-g9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cb8/9540240/a6be3ec14792/fsoa-08-810-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cb8/9540240/d2ddce805ed4/fsoa-08-810-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cb8/9540240/c95170be9233/fsoa-08-810-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cb8/9540240/c8481f9c60af/fsoa-08-810-g4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cb8/9540240/3e0f4e8075ed/fsoa-08-810-g5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cb8/9540240/f4c2cb8f920b/fsoa-08-810-g6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cb8/9540240/ad05dccb123c/fsoa-08-810-g7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cb8/9540240/cdab2a9bbbfd/fsoa-08-810-g8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cb8/9540240/4d92cf1b80bb/fsoa-08-810-g9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cb8/9540240/a6be3ec14792/fsoa-08-810-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cb8/9540240/d2ddce805ed4/fsoa-08-810-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cb8/9540240/c95170be9233/fsoa-08-810-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cb8/9540240/c8481f9c60af/fsoa-08-810-g4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cb8/9540240/3e0f4e8075ed/fsoa-08-810-g5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cb8/9540240/f4c2cb8f920b/fsoa-08-810-g6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cb8/9540240/ad05dccb123c/fsoa-08-810-g7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cb8/9540240/cdab2a9bbbfd/fsoa-08-810-g8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cb8/9540240/4d92cf1b80bb/fsoa-08-810-g9.jpg

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