Elste A, Sullivan M E, Murphy M J
Int J Cell Cloning. 1987 Sep;5(5):385-400. doi: 10.1002/stem.5530050504.
A simplified methodology has been developed which makes it possible to examine the ultrastructural details of cells cloned in vitro while retaining the cell/cell relationships within semi-solid cultures. Using mouse erythropoiesis as the model for study, electron microscopy revealed many normal characteristics of red blood cell differentiation and maturation, as well as several distinct dyserythropoietic features. The usefulness of this technology should apply to fine structural studies of clonally-derived material such as hematopoietic cells, tumor cell lines and primary tumor cultures.
已开发出一种简化方法，该方法能够在保留半固体培养物中细胞与细胞关系的同时，研究体外克隆细胞的超微结构细节。以小鼠红细胞生成作为研究模型，电子显微镜揭示了红细胞分化和成熟的许多正常特征，以及一些明显的异常红细胞生成特征。这项技术的实用性应适用于对克隆来源材料（如造血细胞、肿瘤细胞系和原发性肿瘤培养物）的精细结构研究。
