宫颈上皮细胞中相对端粒长度与高人类乳头瘤病毒载量风险的关联

Association of Relative Telomere Length and Risk of High Human Papillomavirus Load in Cervical Epithelial Cells.

作者信息

Albosale A H, Mashkina E V

机构信息

Department of Medical Laboratory Techniques, Al-Dour Technical Institute, Northern Technical University, Mosul, Iraq.

Genetics Department of Academy of Biology and Biotechnology, Southern Federal University, Rostov-on-Don, Russia.

出版信息

Balkan J Med Genet. 2022 Jun 5;24(2):65-70. doi: 10.2478/bjmg-2021-0026. eCollection 2021 Nov.

DOI:10.2478/bjmg-2021-0026

PMID:36249518

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9524175/

Abstract

Importunate high-risk HPV (HR-HPV) infection is the most common trigger for the cervical carcinogenesis process. In this respect, the presence of cancer can be imputed to telomere lengthening or shortening. This paper explores the possible correlation between relative telomere length and viral load in two groups of women, namely: those with high-risk HPV infection and those who do not have this infection. Thus, samples comprising of 50 women in each group were evaluated for this research. The Amplisens HPV HCR screen-titre-FRT PCR kite was employed for quantitative analysis. Relative telomere length was quantified by real-time PCR. In each of the two HPV load groups, there was no correlation between age and telomere length. Telomere shortening was found in the cervical cell samples of women with high HPV loads, compared with women in the control group. Telomere shortening is associated with elevated HPV loads.

摘要

持续性高危型人乳头瘤病毒（HR-HPV）感染是子宫颈癌发生过程中最常见的诱因。在这方面，癌症的发生可归因于端粒的延长或缩短。本文探讨了两组女性，即高危型HPV感染者和未感染者，其相对端粒长度与病毒载量之间的可能相关性。因此，本研究对每组50名女性的样本进行了评估。采用Amplisens HPV HCR screen-titre-FRT PCR试剂盒进行定量分析。通过实时PCR对相对端粒长度进行定量。在两个HPV载量组中，年龄与端粒长度均无相关性。与对照组女性相比，HPV载量高的女性宫颈细胞样本中发现端粒缩短。端粒缩短与HPV载量升高有关。