State Key Laboratory of Food Science and Technology, International Joint Laboratory on Food Safety, Institute of Analytical Food Safety, School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China.
State Key Laboratory of Food Science and Technology, International Joint Laboratory on Food Safety, Institute of Analytical Food Safety, School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China.
Food Chem. 2023 Mar 15;404(Pt A):134482. doi: 10.1016/j.foodchem.2022.134482. Epub 2022 Oct 1.
Hormone residues in food and drinking water endanger human health, therefore, on-site analysis techniques of superior performance are important for monitoring this risk. In this study, an ultra-sensitive photothermal lateral flow immunoassay (LFIA) for quantification of 17β-estradiol (E) has been developed. Anti-E antibody modified black phosphorus-Au (BP-Au) nanocomposite was developed as a photothermal contrast signal probe and the temperature at test-zone was recorded with an infrared camera. Under the irradiation of 808 nm laser at test-zone, it gave temperatures negatively related to the concentrations of E in samples. Under optimal detecting conditions, the developed photothermal LFIA exhibited a limit of detection of 50 pg mL, over 100-fold more sensitive than visual LFIA, and a linear range of 3 orders of magnitude. This method has been successfully applied to water, milk, and milk powder samples.
食物和饮水中的激素残留会危害人类健康，因此，具有优越性能的现场分析技术对于监测这种风险非常重要。本研究开发了一种用于定量检测 17β-雌二醇 (E) 的超灵敏光热侧向流动免疫分析 (LFIA)。将抗 E 抗体修饰的黑磷-金 (BP-Au) 纳米复合材料作为光热对比信号探针，并用红外摄像机记录测试区的温度。在测试区 808nm 激光的照射下，它给出的温度与样品中 E 的浓度呈负相关。在最佳检测条件下，所开发的光热 LFIA 的检测限为 50pgmL，比视觉 LFIA 灵敏 100 多倍，线性范围为 3 个数量级。该方法已成功应用于水、牛奶和奶粉样品的检测。
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