Department of Nursing, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.
Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.
J Vasc Access. 2024 Mar;25(2):615-624. doi: 10.1177/11297298221118742. Epub 2022 Oct 17.
To demonstrate the equivalence and substitutability of two blood collection methods: the push-pull method from a CVC and direct venous puncture (DVP).
A comparative, within-subject study was conducted between September 2021 and December 2021 at a hospital in NanTong city. The sample comprised critically ill patients aged 18 and older in critical care units such as general, emergent, cardiac, respiratory, and neurological units. A total of 154 paired blood samples were collected via a CVC and direct venous puncture. This study focused on the laboratory results of the coagulation and hematologic tests. The reproducibility and reliability of the results were calculated by the mean of the coefficient of variation (CV) and the intraclass correlation coefficient (ICC). Bland-Altman statistics were used to analyze the substitutability of the two blood collection methods.
The difference in the means between the two methods ranged from -1.61 to 0.09, and the coefficients of variation for both methods were similar. The ICCs of the two methods were all above 0.90, which indicated excellent reliability. In the Bland-Altman plots, all of the blood samples that obtained by the push-pull method were within clinically acceptable ranges compared to the samples obtained by direct venous puncture.
The push-pull method of collecting blood specimens from a CVC should be acceptable for coagulation and hematologic laboratory tests.
证明两种采血方法的等效性和可替代性：从 CVC 推挽法和直接静脉穿刺（DVP）采血。
2021 年 9 月至 12 月在南通市一家医院进行了一项比较性、受试者内研究。该样本包括重症监护病房（如普通、急诊、心脏、呼吸和神经科病房）的 18 岁及以上危重病患者。通过 CVC 和直接静脉穿刺共采集了 154 对血液样本。本研究主要关注凝血和血液学检测的实验室结果。结果的再现性和可靠性通过变异系数（CV）和组内相关系数（ICC）的平均值来计算。 Bland-Altman 统计分析用于分析两种采血方法的可替代性。
两种方法之间的均值差异范围为-1.61 至 0.09，两种方法的 CV 相似。两种方法的 ICC 均高于 0.90，表明可靠性良好。在 Bland-Altman 图中，与直接静脉穿刺获得的样本相比，通过推挽法获得的所有血液样本均在临床可接受范围内。
从 CVC 采集血标本的推挽法应适用于凝血和血液学实验室检测。
