电针可减轻脓毒症小鼠的认知功能障碍和神经元焦亡。
Electroacupuncture alleviates cognitive dysfunction and neuronal pyroptosis in septic mice.
作者信息
Li Yan, Li Zhaoying, He Fujuan, Qin Chenguang, Fan Rui, Zhang Fangxiang, Wang Bin
机构信息
Department of Anesthesiology, Guizhou Provincial People's Hospital, Guiyang, China.
出版信息
Acupunct Med. 2023 Aug;41(4):246-256. doi: 10.1177/09645284221117847. Epub 2022 Oct 18.
BACKGROUND
Sepsis is defined as organ dysfunction caused by an uncontrolled response to infection and is followed by a high incidence of cognitive dysfunction, which can severely affect patients' quality of life. Previous studies have suggested that electroacupuncture (EA) is protective against sepsis-associated cognitive dysfunction and that pyroptosis plays a vital role in cognitive function. The aim of this study was to investigate the effect of EA on cognition and neuronal pyroptosis in a mouse model of sepsis.
METHODS
Sepsis was induced by cecal ligation and puncture (CLP) surgery. Mice were randomly divided into three groups (control, CLP and CLP + EA). EA was performed at bilateral ST36 for three consecutive days after the surgery. The 7-day survival rate of each group was observed on the seventh day after the surgery. The Morris water maze (MWM) was used to test cognitive function from the 8th to 12th day after the surgery. We used transmission electron microscopy (TEM) and transferase dUTP nick-end labeling (TUNEL) staining to determine the structural integrity of hippocampal neuronal membranes and the number of surviving neurons in the hippocampal tissues, respectively. Expression of nucleotide-binding domain-like receptor protein 1 (NLRP1), caspase-1 and gasdermin-D (GSDM D) in hippocampal CA1 neurons was detected by Western blotting and real-time fluorescence quantitative polymerase chain reaction (RT-qPCR), and caspase-1 concentrations were measured by enzyme-linked immunosorbent assay.
RESULTS
Compared with the CLP group, 7-day survival rates and cognitive function were significantly improved in the CLP + EA group. After EA treatment, the integrity of the hippocampal CA1 neuronal membrane and mortality of hippocampal neurons were significantly decreased, and expression of NLRP1, caspase-1 and GSDM D was downregulated.
CONCLUSION
EA can alleviate cognitive dysfunction and neuronal pyroptosis in septic mice.
背景
脓毒症被定义为由对感染的失控反应引起的器官功能障碍，随后认知功能障碍的发生率很高，这会严重影响患者的生活质量。先前的研究表明，电针（EA）对脓毒症相关的认知功能障碍具有保护作用，并且细胞焦亡在认知功能中起重要作用。本研究的目的是探讨电针在脓毒症小鼠模型中对认知和神经元细胞焦亡的影响。
方法
通过盲肠结扎和穿刺（CLP）手术诱导脓毒症。将小鼠随机分为三组（对照组、CLP组和CLP + EA组）。术后连续三天在双侧足三里进行电针治疗。术后第七天观察每组的7天生存率。术后第8至12天使用莫里斯水迷宫（MWM）测试认知功能。我们分别使用透射电子显微镜（TEM）和末端脱氧核苷酸转移酶介导的缺口末端标记（TUNEL）染色来确定海马神经元膜的结构完整性和海马组织中存活神经元的数量。通过蛋白质免疫印迹法和实时荧光定量聚合酶链反应（RT-qPCR）检测海马CA1神经元中核苷酸结合寡聚化结构域样受体蛋白1（NLRP1）、半胱天冬酶-1和gasdermin-D（GSDM D）的表达，并通过酶联免疫吸附测定法测量半胱天冬酶-1的浓度。
结果
与CLP组相比，CLP + EA组的7天生存率和认知功能显著改善。电针治疗后，海马CA1神经元膜的完整性和海马神经元的死亡率显著降低，NLRP1、半胱天冬酶-1和GSDM D的表达下调。
结论
电针可减轻脓毒症小鼠的认知功能障碍和神经元细胞焦亡。
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