School of Nano Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal 721302, India.
School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal 721302, India.
Colloids Surf B Biointerfaces. 2022 Dec;220:112926. doi: 10.1016/j.colsurfb.2022.112926. Epub 2022 Oct 13.
In recent times, carbon dots (CDs) are emerging for numerous interdisciplinary applications by modulating their inherent chemical functionality during or post-synthesis modification. The current study reports the hydrothermal synthesis of polyvinylpyrrolidone K-30 (PVP) passivated clove bud-derived carbon dots (PPCCDs) for multifaceted applications. The adopted technique is facile and environmentally friendly for the production of CDs with in situ PVP passivation. Physicochemical characterization of CDs is performed using various spectroscopic and microscopic techniques. The study reveals the formation of nitrogen-doped spherical PPCCDs with an average hydrodynamic size of ∼ 4.9 nm. It is also evident that there is modulation in optical properties and quantum efficiency as a result of PVP passivation. The study further demonstrates their suitability in biological environments as observed by pH stability, photostability, and cytocompatibility results. PPCCDs have shown significant antioxidant activity against DPPH (EC: 57 µg/mL), suppression of superoxide anion radical (EC: 53 µg/mL), and an efficient catalytic activity towards degradation of Rhodamine-B (Rh-B) dye. UV-Visible spectroscopy unveil the reaction mechanism during antioxidant and catalytic activities of CDs that are validated by Electron paramagnetic resonance (EPR) spectroscopy with an indication of effective electron or proton donating abilities. Its bioimaging potential is evidenced through cellular fluorescence imaging with 3T3 and L929 cell lines.
近年来,通过在合成过程中或之后对其固有化学功能进行调节,碳点 (CDs) 在许多跨学科应用中崭露头角。本研究报告了通过水热合成法,用聚维酮 K-30 (PVP) 对丁香芽衍生的碳点 (PPCCDs) 进行钝化,以实现多方面的应用。所采用的技术简便且环保,可用于原位 PVP 钝化的 CDs 生产。通过各种光谱和显微镜技术对 CDs 的物理化学特性进行了表征。研究表明,形成了具有平均水动力直径约为 4.9nm 的氮掺杂球形 PPCCDs。此外,由于 PVP 的钝化,还可以观察到光学性质和量子效率的调制。通过 pH 稳定性、光稳定性和细胞相容性结果进一步证明了它们在生物环境中的适用性。PPCCDs 对 DPPH(EC:57µg/mL)具有显著的抗氧化活性,对超氧阴离子自由基(EC:53µg/mL)具有抑制作用,对 Rhodamine-B(Rh-B)染料的降解具有高效的催化活性。紫外-可见光谱揭示了 CDs 抗氧化和催化活性过程中的反应机制,这一机制得到了电子顺磁共振(EPR)光谱的验证,并表明了其有效提供电子或质子的能力。通过 3T3 和 L929 细胞系的细胞荧光成像证明了其生物成像的潜力。
Colloids Surf B Biointerfaces. 2022-12
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