Kuo Tsung-Rong, Sung Shuo-Yuan, Hsu Chun-Wei, Chang Chih-Jui, Chiu Tai-Chia, Hu Cho-Chun
Ph. D. Program in Graduate Institute of Nanomedicine and Medical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei, 11031, Taiwan.
Department of Applied Science, National Taitung University, Taitung, 95002, Taiwan.
Anal Bioanal Chem. 2016 Jan;408(1):77-82. doi: 10.1007/s00216-015-9138-8. Epub 2015 Oct 29.
One-pot green synthesis of fluorescent nitrogen-doped carbon nanodots (CNDs) was developed by hydrothermal treatments of biocompatible polyvinylpyrrolidone (PVP) and glycine. The fluorescent nitrogen-doped CNDs exhibited excellent water solubility, low cytotoxicity, and good salt stability for biological imaging. UV-vis spectroscopy, fluorescence spectroscopy, transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR) spectroscopy, and Raman spectroscopy were applied to confirm the optical and structural characteristics of the CNDs. Fluorescence of the CNDs was tunable from 417 to 450 nm adjusted by different excitation energy. Fluorescent quantum yield of the CNDs (21.43%) was significantly increased ~47.59% in comparison to that of the CNDs (14.52%) without nitrogen doping by glycine. In the in vivo imaging system (IVIS), fluorescence signal of the nitrogen-doped CNDs was obviously observed in the lungs at 12- and 24-h post-injection. Our work has shown the potential applications of the nitrogen-doped CNDs in fluorescence imaging in vivo.
通过对生物相容性聚乙烯吡咯烷酮（PVP）和甘氨酸进行水热处理，开发了一种一锅法绿色合成荧光氮掺杂碳纳米点（CNDs）的方法。荧光氮掺杂碳纳米点在生物成像方面表现出优异的水溶性、低细胞毒性和良好的盐稳定性。采用紫外可见光谱、荧光光谱、透射电子显微镜（TEM）、傅里叶变换红外光谱（FTIR）和拉曼光谱来确认碳纳米点的光学和结构特征。通过不同的激发能量，碳纳米点的荧光可在417至450nm范围内调节。与未用甘氨酸进行氮掺杂的碳纳米点（14.52%）相比，氮掺杂碳纳米点的荧光量子产率（21.43%）显著提高了约47.59%。在体内成像系统（IVIS）中，在注射后12小时和24小时时，在肺部明显观察到氮掺杂碳纳米点的荧光信号。我们的工作展示了氮掺杂碳纳米点在体内荧光成像中的潜在应用。
