苦参碱通过 microRNA-299-3p/PGAM1 轴诱导肝癌细胞凋亡，抑制 EMT 和干性。

Matrine induces hepatocellular carcinoma apoptosis and represses EMT and stemness through microRNA-299-3p/PGAM1 axis.

机构信息

Department of Pharmacy, The First Affiliated Hospital of Kangda College of Nanjing Medical University/The First People's Hospital of Lianyungang, Lianyungang City, Jiangsu Province, China.

Department of Rehabilitation Medicine, The First Affiliated Hospital of Kangda college of Nanjing Medical University/The First People's Hospital of Lianyungang, Lianyungang City, Jiangsu Province, China.

出版信息

Growth Factors. 2022 Nov;40(5-6):200-211. doi: 10.1080/08977194.2022.2113073. Epub 2022 Oct 19.

DOI:10.1080/08977194.2022.2113073

PMID:36260520

Abstract

This study explored the impacts of matrine on hepatocellular carcinoma (HCC) cell growth, metastasis, epithelial-mesenchymal transition (EMT), and stemness through regulating the microRNA / () axis. The association between expression with the prognosis of HCC patients was studied. and sequences were transfected into matrine-treated HCC cells, and cell proliferation, invasion, apoptosis, and stemness were detected, as well as protein expression of EMT- and stemness-related makers. The targeting relationship between and was identified. Matrine elevated expression, repressed proliferation, invasion, and anti-apoptosis of HCC cells, and constrained EMT and stemness . was a target of . Repression of rescued the effects of downregulation on HCC cells. Matrine stimulates HCC cell apoptosis and represses the process of EMT and stemness through the / axis.

摘要

本研究通过调控 microRNA/()轴探讨了苦参碱对肝癌（HCC）细胞生长、转移、上皮-间充质转化（EMT）和干性的影响。研究了表达与 HCC 患者预后的关系。将和序列转染到苦参碱处理的 HCC 细胞中，检测细胞增殖、侵袭、凋亡和干性，以及 EMT 和干性相关标志物的蛋白表达。鉴定了与之间的靶向关系。苦参碱升高表达，抑制 HCC 细胞的增殖、侵袭和抗凋亡，并抑制 EMT 和干性。是的靶点。下调的抑制作用被 rescue。苦参碱通过/轴刺激 HCC 细胞凋亡并抑制 EMT 和干性的发生。