High Technology Application and Research Centre, Erzurum Technical University, Erzurum, Turkey.
Kahta Vocational School, Adıyaman University, Adıyaman, Turkey.
Prep Biochem Biotechnol. 2023;53(1):101-107. doi: 10.1080/10826068.2022.2134148. Epub 2022 Oct 20.
Jerusalem artichoke (JA) is a nutritional vegetable for human diet depending on its natural structure, especially high inulin content and it is the second inulin source for commercial production in the world, after chicory. It was aimed to investigate the inulinase production capability of TS61 (GenBank accession: MN749818) using JA as an economical and effective substrate comparing with the pure chicory inulin and to optimize the fermentation using Taguchi design of experiment (DOE) in this study. Besides, the effects of sucrose on inulinase production either combined with JA or in its absence were also studied. Taguchi L16 orthogonal array was employed for optimization. Both of inulinase activities obtained from JA and pure inulin gave the maximum result at the 10th experimental run as 40.21 U/mL and 57.35 U/mL, respectively. The optimum levels were detected for each factor as, 30 g/L JA, 30 g/L sucrose, pH 5.5, and four days for time. The predicted value was found as 41.63 U/mL that was similar to the obtained result as 41.17 U/mL. Finally, inulinase activity was increased approximately 8-folds after optimization. The sucrose-free medium had similar effects with higher concentrations of JA at long incubation time. This is the first investigation about inulinase production by .
洋姜（JA）是一种营养丰富的蔬菜，可作为人类的食物，这主要依赖于其天然结构，尤其是其含有丰富的菊粉。JA 是世界上继菊苣之后第二大商业化生产的菊粉来源，可作为生产菊粉酶的经济有效底物。本研究旨在探讨利用 JA 作为经济有效的底物生产菊粉酶的能力，并与纯菊苣菊粉进行比较，同时利用田口设计实验（DOE）对发酵进行优化。此外，还研究了蔗糖对 JA 存在或不存在时菊粉酶生产的影响。采用 Taguchi L16 正交数组进行优化。从 JA 和纯菊苣菊粉中获得的菊粉酶活性在第 10 次实验运行中均达到最大值，分别为 40.21 U/mL 和 57.35 U/mL。检测到每个因素的最佳水平分别为：30 g/L JA、30 g/L 蔗糖、pH5.5 和 4 天。预测值为 41.63 U/mL，与获得的 41.17 U/mL 结果相似。最终，优化后菊粉酶活性增加了约 8 倍。在较长的孵育时间内，无蔗糖培养基与较高浓度的 JA 具有相似的效果。这是首次对. 生产菊粉酶进行的研究。
