Valadas Jéssica, Sachett Adrieli, Marcon Matheus, Bastos Leonardo M, Piato Angelo
Programa de Pós-Graduação em Neurociências, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos, 2600, Prédio UFRGS n° 21116, 6º andar - Campus Saúde, Porto Alegre, RS, 90035-003, Brazil.
Departamento de Bioquímica, Farmacologia e Fisiologia, Universidade Federal do Triângulo Mineiro (UFTM), Uberaba, MG, Brazil.
Environ Sci Pollut Res Int. 2023 Feb;30(8):21144-21155. doi: 10.1007/s11356-022-23692-4. Epub 2022 Oct 20.
Ochratoxin A (OTA) is a mycotoxin produced by species of filamentous fungi widely found as a contaminant in food and with high toxic potential. Studies have shown that this toxin causes kidney and liver damage; however, data on the central nervous system effects of exposure to OTA are still scarce. Thus, this study aimed to investigate the effects of exposure to OTA on behavioral and neurochemical parameters in adult zebrafish. The animals were treated with different doses of OTA (1.38, 2.77, and 5.53 mg/kg) with intraperitoneal injections and submitted to behavioral evaluations in the open tank and social interaction tests. Subsequently, they were euthanized, and the brains were used to assess markers associated with oxidative status. In the open tank test, OTA altered distance traveled, absolute turn angle, mean speed, and freezing time. However, no significant effects were observed in the social interaction test. Moreover, OTA also increased glutathione peroxidase (GPx), glutathione-S-transferase (GST), and glutathione reductase (GR) levels and decreased non-protein thiols (NPSH) levels in the zebrafish brain. This study showed that OTA can affect behavior and neurochemical levels in zebrafish.
赭曲霉毒素A（OTA）是一种由丝状真菌产生的霉菌毒素，在食品中广泛存在，是一种污染物，具有很高的毒性潜力。研究表明，这种毒素会导致肾脏和肝脏损伤；然而，关于接触OTA对中枢神经系统影响的数据仍然很少。因此，本研究旨在调查接触OTA对成年斑马鱼行为和神经化学参数的影响。通过腹腔注射，用不同剂量的OTA（1.38、2.77和5.53mg/kg）对动物进行处理，并在开放水箱和社交互动测试中对其进行行为评估。随后，对它们实施安乐死，并使用大脑来评估与氧化状态相关的标志物。在开放水箱测试中，OTA改变了游动距离、绝对转弯角度、平均速度和静止时间。然而，在社交互动测试中未观察到显著影响。此外，OTA还增加了斑马鱼大脑中谷胱甘肽过氧化物酶（GPx）、谷胱甘肽-S-转移酶（GST）和谷胱甘肽还原酶（GR）的水平，并降低了非蛋白质巯基（NPSH）的水平。本研究表明，OTA会影响斑马鱼的行为和神经化学水平。
