Enrione E B, Ogle C, Smith J E, Popp M B
J Surg Res. 1987 Aug;43(2):149-57. doi: 10.1016/0022-4804(87)90158-2.
Assessment of visceral and somatic protein stores by serum proteins in surgical oncology patients has been difficult to interpret. Tumor-bearing (methylcholanthrene sarcoma) (TB) and nontumor-bearing (NTB) Fischer 344 rats were infused with a total parenteral nutrition solution at either 25, 100, or 175% of an infusion rate that provided sufficient nutrients for normal rat growth: On the fifth day after diet initiation, the rats were exsanguinated, decapitated, eviscerated, and skinned. Serum was analyzed for transferrin (TF), complement C3, albumin (ALB), retinol-binding protein (RBP), and transthyretin (TTR). Carcass (CAR) and organ (VIS) nitrogens were determined by the Kjeldahl method. TTR, TF, and RBP correlated significantly with TB and NTB VIS nitrogen and TB CAR nitrogen. The correlation of NTB VIS nitrogen with TTR, TF, and RBP (r range = 0.70-0.85, P less than 0.001) was higher than for TB rats (r range = 0.53-0.57, P less than 0.005). The correlation of TB carcass nitrogen (r range = 0.47-0.51, P less than 0.01) with TTR, TF, and RBP was higher than for NTB carcass nitrogen which was not significant (r range = 0.25-0.37, P less than 0.57). These data indicate that TTR, TF, and RBP do correlate with components of body nitrogen mass, but factors other than nutrition may influence their metabolism in the TB host.
通过血清蛋白评估外科肿瘤患者的内脏和躯体蛋白质储备一直难以解读。给荷瘤（甲基胆蒽肉瘤）（TB）和无瘤（NTB）的Fischer 344大鼠输注全胃肠外营养溶液，输注速率分别为正常大鼠生长所需充足营养的25%、100%或175%：在开始饮食后的第5天，将大鼠放血、断头、取出内脏并剥皮。分析血清中的转铁蛋白（TF）、补体C3、白蛋白（ALB）、视黄醇结合蛋白（RBP）和甲状腺素转运蛋白（TTR）。采用凯氏定氮法测定胴体（CAR）和器官（VIS）中的氮含量。TTR、TF和RBP与TB和NTB的VIS氮以及TB的CAR氮显著相关。NTB的VIS氮与TTR、TF和RBP的相关性（r范围=0.70 - 0.85，P<0.001）高于TB大鼠（r范围=0.53 - 0.57，P<0.005）。TB胴体氮与TTR、TF和RBP的相关性（r范围=0.47 - 0.51，P<0.01）高于NTB胴体氮，后者不显著（r范围=0.25 - 0.37，P<0.57）。这些数据表明，TTR、TF和RBP确实与身体氮质量的组成部分相关，但营养以外的因素可能会影响它们在TB宿主中的代谢。
