Chen Qiang, Tang Keren, Luo Dengwang, Han Luodan, Yu ChunXiao, Shen Yiping, Lin Qi, Chen Yiting, Li Chunyan, Chen Jinghua, Lan Jianming
College of Materials and Chemical Engineering, MinJiang University, Fuzhou, China.
The School of Pharmacy, Fujian Medical University, Fuzhou, China.
Front Chem. 2022 Oct 4;10:1028441. doi: 10.3389/fchem.2022.1028441. eCollection 2022.
Based on the mechanism of luminescence resonance energy transfer (LRET) and using a special single strand DNA as the recognition element, a portable paper-based sensor for the accurate detection of total heavy rare-earth ions (mainly Gd, Tb and Dy) concentration was proposed. The RNA cleaving-DNAzyme should recognize rare-earth ions to cleave RNA on DNA duplexes linking UCNPs and AuNPs, causing UCNPs and AuNPs to approach each other, inducing LRET, which attenuated the green upconversion luminescence (UCL) triggered by the 980 nm laser. UCL was captured by a charge-coupled device (CCD) image sensor and processed with the red-green-blue (RGB) image to quantitatively analyze heavy rare-earth ions in the samples. In the range of 5-50 μmol·L-1, the sensor has good sensitivity, with the limit of detection of 1.26 μmol L.
基于发光共振能量转移（LRET）机制，以特殊单链DNA作为识别元件，提出了一种用于准确检测总重稀土离子（主要为Gd、Tb和Dy）浓度的便携式纸质传感器。RNA切割型DNAzyme应识别稀土离子，切割连接上转换纳米粒子（UCNPs）和金纳米粒子（AuNPs）的DNA双链上的RNA，使UCNPs和AuNPs相互靠近，引发LRET，从而减弱980 nm激光激发的绿色上转换发光（UCL）。UCL由电荷耦合器件（CCD）图像传感器捕获，并用红-绿-蓝（RGB）图像进行处理，以定量分析样品中的重稀土离子。在5 - 50 μmol·L-1范围内，该传感器具有良好的灵敏度，检测限为1.26 μmol L。
