Smith K, Chandler R L
Vet Pathol. 1978 Sep;15(5):638-48. doi: 10.1177/030098587801500507.
Streptococcal mastitis was produced experimentally in mice inoculated by the intramammary route; freeze-etched preparations from the affected mammary glands were studied by electron microscopy. The inoculated cocci were seen free in the acinar lumen, within luminal phagocytes and within cells of the epithelium. No significant pathological changes were noted in the junctional complexes between secretory epithelial cells. The results were comparable to those obtained by ultrathin sectioning and indicated that, while cocci can transfer from the acinar lumen into the substance of the epithelium and towards a subepithelial location, the junctional complexes between epithelial cells present a potential barrier to movement through the intercellular spaces.
通过乳腺途径接种的小鼠被实验性诱发了链球菌性乳腺炎；对来自受影响乳腺的冷冻蚀刻制剂进行了电子显微镜研究。接种的球菌可见于腺泡腔内、腔内吞噬细胞内和上皮细胞内。在分泌上皮细胞之间的连接复合体中未观察到明显的病理变化。结果与通过超薄切片获得的结果相当，表明虽然球菌可以从腺泡腔转移到上皮实质并朝着上皮下位置移动，但上皮细胞之间的连接复合体对通过细胞间隙的移动构成了潜在障碍。
