Wang Xiaobo, Wu Zhansheng, Xiang Huichun, He Yanhui, Zhu Shuangxi, Zhang Ziyan, Li Xueping, Wang Jianwen
Xi'an Key Laboratory of Textile Chemical Engineering Auxiliaries, School of Environmental and Chemical Engineering, Xi'an Polytechnic University, Xi'an, 710048, People's Republic of China.
Environ Sci Pollut Res Int. 2023 Feb;30(8):21548-21564. doi: 10.1007/s11356-022-23564-x. Epub 2022 Oct 22.
The genus Enterobacter is widely recognized for its biotechnology potential in improving soil environment and crop growth promotion. To further explore these biotechnological potentials, we sequenced and analyzed the whole genome of Enterobacter cloacae Rs-2. The analysis showed that the total length of the Rs-2 genome was 6,965,070,514 bp, and GC content was 55.80%; the annotation results of GO and COG databases showed that the genome contains a variety of growth-promoting genes, such as iscU, glnA, glnB (nitrogen fixation); iucABCD (siderophore synthesis) and fepA, fcuA, fhuA, and pfeA, etc. (siderophore transport); ipdC (secreted IAA) and gcd, pqqBCDEF (dissolved phosphorus), etc. No pathogenic factors such as virulence genes were found. The application of Rs-2 as a soil inoculant in pot experiments showed great potential for growth promotion. This study proved the plant growth-promoting ability of Rs-2 at the molecular level through genetic screening and analysis, which provided guidance for the further improvement of the strain and laid a foundation for its application in agricultural production.
肠杆菌属因其在改善土壤环境和促进作物生长方面的生物技术潜力而被广泛认可。为了进一步探索这些生物技术潜力，我们对阴沟肠杆菌Rs-2的全基因组进行了测序和分析。分析表明，Rs-2基因组全长为6,965,070,514 bp，GC含量为55.80%；GO和COG数据库的注释结果表明，该基因组包含多种促进生长的基因，如iscU、glnA、glnB（固氮）；iucABCD（铁载体合成）以及fepA、fcuA、fhuA和pfeA等（铁载体转运）；ipdC（分泌吲哚-3-乙酸）和gcd、pqqBCDEF（溶解磷）等。未发现毒力基因等致病因素。在盆栽试验中，将Rs-2作为土壤接种剂应用显示出巨大的促生长潜力。本研究通过基因筛选和分析在分子水平上证明了Rs-2促进植物生长的能力，为该菌株的进一步改良提供了指导，并为其在农业生产中的应用奠定了基础。
