USP7 and SET9 Expression in The Oligospermic Human Semen: A Case-Control Study.

BACKGROUND

Oligospermia is defined as a less than 15 million per milliliter sperm in each ejaculation of semen. Proper and complete spermatogenesis requires the expression of a large number of genes. As a result, stopping the expression of any of them may lead to disrupt the process of spermatogenesis. In order to understand the disorders of spermatogenesis, it is necessary to study expression of effective genes in the spermatogenesis process. Therefore, in the present study, and gene expression was examined in the healthy and oligospermic men.

MATERIALS AND METHODS

In this case-control study, semen samples of individuals with normal sperm and oligospermia were collected from men who referred to the Roya Clinic (Qom, Iran) according to World Health Organization (WHO) parameters after obtaining consent. Then the expression of and genes in two groups was analyzed using quantitative polymerase chain reaction (qPCR).

RESULTS

There was no difference forage between the healthy and oligospermic individuals (P=0.889). The data showed that, gene expression in the patients was 3.99 times higher than the control group (P<0.001). The expression of gene in the patient was 1.28 times less than the control group, which was not significant (P=0.231). The results indicated that gene expression was increased in the 84% of oligospermic individuals.

CONCLUSION

The gene can be considered as one of the molecular markers in the development of oligospermia.