肝内胆管癌中环状RNA的表达谱及竞争性内源性RNA网络
The expression profiles of circular RNAs and competing endogenous RNA networks in intrahepatic cholangiocarcinoma.
作者信息
Liang Zi, Liu Liyan, Guo Xinyi, Wu Xia, Yu Yun-Li, Yu Ziyang, Hu Xiaolong, Zhang Xing, Wang Ji
机构信息
Department of Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, China.
School of Biology and Basic Medical Science, Soochow University, Suzhou, China.
出版信息
Front Cell Dev Biol. 2022 Oct 7;10:942853. doi: 10.3389/fcell.2022.942853. eCollection 2022.
Intrahepatic cholangiocarcinoma (iCCA) is a heterogeneous entity with diverse etiologies, morphologies, and clinical outcomes, but our knowledge of its epidemiology and carcinogenesis is very limited. The expression patterns of circRNAs were explored in iCCA tissues and corresponding adjacent normal ones, denoted by (iCCA) and (iCCAP), respectively, using high-throughput sequencing. A total of 117 differential expressed (DE) circRNAs were identified. Based on the parental transcripts of circRNAs, these DE circRNAs were related to several important GO terms and were enriched in important pathways. Two circRNA-mediated ceRNA networks were constructed and many important metabolic pathways related to mRNAs were regulated by DE circRNAs miRNAs. Our study revealed the DE circRNAs in the iCCA tissues compared with iCCAP ones, suggesting that circRNAs may play crucial roles in the pathogenesis of iCCA.
肝内胆管癌（iCCA）是一种具有多种病因、形态和临床结局的异质性疾病，但我们对其流行病学和致癌机制的了解非常有限。本研究利用高通量测序技术，分别在肝内胆管癌组织（iCCA）及其相应的癌旁正常组织（iCCAP）中探索环状RNA（circRNA）的表达模式。共鉴定出117条差异表达的circRNA。基于circRNA的亲本转录本，这些差异表达的circRNA与多个重要的基因本体（GO）术语相关，并富集于重要的信号通路中。构建了两个circRNA介导的竞争性内源RNA（ceRNA）网络，许多与mRNA相关的重要代谢通路受差异表达的circRNA-miRNA调控。我们的研究揭示了与癌旁正常组织相比，肝内胆管癌组织中的差异表达circRNA，提示circRNA可能在肝内胆管癌的发病机制中发挥关键作用。
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