Kumar Prashant, Mishra Ankita, Rahim Mohammed Saba, Sharma Vinita, Madhawan Akansha, Parveen Afsana, Fandade Vikas, Sharma Himanshu, Roy Joy
Agri-Food Biotechnology Division, National Agri-Food Biotechnology Institute, Mohali, Punjab 140306 India.
Regional Centre for Biotechnology, NCR Biotech Science Cluster, 3rd Milestone Gurgaon-Faridabad Expressway, Faridabad, 121001 India.
3 Biotech. 2022 Nov;12(11):295. doi: 10.1007/s13205-022-03364-3. Epub 2022 Sep 28.
High amylopectin starch is an important modified starch for food processing industries. Despite a thorough understanding of starch biosynthesis pathway, the regulatory mechanism responsible for amylopectin biosynthesis is not well explored. The present study utilized transcriptome sequencing approach to understand the molecular basis of high amylopectin content in three high amylopectin mutant wheat lines ('TAC 6', 'TAC 358', and 'TAC 846') along with parent variety 'C 306'. Differential scanning calorimetry (DSC) of high amylopectin starch identified a high thermal transition temperature and scanning electron microscopy (SEM) revealed more spherical starch granules in mutant lines compared to parent variety. A set of 4455 differentially expressed genes (DEGs) were identified at two-fold compared to the parent variety in high amylopectin wheat mutants. At ten-fold, 279 genes, including two starch branching genes ( and ), were up-regulated and only 30 genes, including the starch debranching enzyme (), were down-regulated. Among the genes, different isoforms of sucrose non-fermenting-1-related protein kinase-1 (-3B and -3D) and its regulatory subunit, sucrose non-fermenting-4 (-2A, -2B, and -5D), were found to be highly up-regulated. Further, expression of the DEGs related to starch biosynthesis pathway and and was performed using qRT-PCR. High expression of , , and SBEII isoforms suggests their probable role in high amylopectin starch biosynthesis in grain endosperm.
The online version contains supplementary material available at 10.1007/s13205-022-03364-3.
高支链淀粉是食品加工业中一种重要的改性淀粉。尽管对淀粉生物合成途径有深入了解，但负责支链淀粉生物合成的调控机制尚未得到充分探索。本研究利用转录组测序方法，以亲本品种“C 306”为对照，了解三个高支链淀粉突变小麦品系（“TAC 6”、“TAC 358”和“TAC 846”）中高支链淀粉含量的分子基础。高支链淀粉的差示扫描量热法（DSC）显示其热转变温度较高，扫描电子显微镜（SEM）显示突变品系中的淀粉颗粒比亲本品种的更呈球形。与亲本品种相比，在高支链淀粉小麦突变体中鉴定出一组4455个差异表达基因（DEG）。在10倍差异时，279个基因上调，包括两个淀粉分支基因（和），只有30个基因下调，包括淀粉脱支酶（）。在这些基因中，发现蔗糖非发酵-1相关蛋白激酶-1的不同异构体（-3B和-3D）及其调节亚基蔗糖非发酵-4（-2A、-2B和-5D）高度上调。此外，使用qRT-PCR对与淀粉生物合成途径以及和相关的DEG进行了表达分析。、和SBEII异构体的高表达表明它们可能在籽粒胚乳高支链淀粉生物合成中发挥作用。
在线版本包含可在10.1007/s13205-022-03364-3获取的补充材料。
