Tsai Shiao-Wen, Hsu Yu-Wei, Pan Whei-Lin, Vadivelmurugan Adhisankar, Hwang Pai-An, Hsu Fu-Yin
Department of Biomedical Engineering, Chang Gung University, Taoyuan 33302, Taiwan.
Department of Periodontics, Chang Gung Memorial Hospital, Taipei 10507, Taiwan.
J Funct Biomater. 2022 Sep 29;13(4):168. doi: 10.3390/jfb13040168.
Synthetic hydroxyapatite has good biocompatibility, bioactivity and osteoconductive ability because its chemical properties and biological properties are similar to those of bioapatite in bone tissue. Strontium-substituted hydroxyapatite has better degradability than hydroxyapatite and can both promote osteogenesis and inhibit adipogenesis in mesenchymal stem cells. Hence, hydroxyapatite and strontium-substituted hydroxyapatite are widely used as bone graft materials, cell carriers and drug/gene delivery carriers. In addition, osteoblasts cultured on aligned nanofibrous substrates had higher expression of osteogenesis-related genes than did those cultured on random nanofibrous substrates. However, to date, no study has explored the effects of the components and orientation of hydroxyapatite nanofibrous substrates on osteoblastic behavior. In this study, a random hydroxyapatite nanofibrous substrate (R-HANF), a random strontium-substituted hydroxyapatite nanofibrous substrate (R-SrHANF), an aligned hydroxyapatite nanofibrous substrate (A-HANF) and an aligned strontium-substituted hydroxyapatite nanofibrous substrate (A-SrHANF) were successfully fabricated by using the electrospinning technique. The effect of fiber composition on osteoblast-like MG63 cells was assessed by evaluating cell morphology, cell proliferation and osteogenesis-related gene expression. The results showed that MG63 cells cultured on A-SrHANF had higher osteogenesis-related gene expression than those cultured on A-HANF. Additionally, MG63 cells were cultured on R-SrHANF and A-SrHANF to evaluate the effects of fiber orientation on cell behavior. On A-SrHANF, the cells aligned along the direction of the nanofibers, with typical bipolar morphologies, and exhibited higher osteogenesis-related gene expression than cells on R-SrHANF. Hence, the components and orientation of hydroxyapatite nanofibrous substrates are critical parameters affecting the osteogenesis process.
合成羟基磷灰石具有良好的生物相容性、生物活性和骨传导能力，因为其化学性质和生物学性质与骨组织中的生物磷灰石相似。锶取代的羟基磷灰石比羟基磷灰石具有更好的降解性，并且既能促进间充质干细胞的成骨作用，又能抑制其成脂作用。因此，羟基磷灰石和锶取代的羟基磷灰石被广泛用作骨移植材料、细胞载体和药物/基因递送载体。此外，在排列的纳米纤维基质上培养的成骨细胞比在随机纳米纤维基质上培养的成骨细胞具有更高的成骨相关基因表达。然而，迄今为止，尚无研究探讨羟基磷灰石纳米纤维基质的成分和取向对成骨细胞行为的影响。在本研究中，通过静电纺丝技术成功制备了随机羟基磷灰石纳米纤维基质（R-HANF）、随机锶取代羟基磷灰石纳米纤维基质（R-SrHANF）、排列的羟基磷灰石纳米纤维基质（A-HANF）和排列的锶取代羟基磷灰石纳米纤维基质（A-SrHANF）。通过评估细胞形态、细胞增殖和成骨相关基因表达来评价纤维成分对成骨样MG63细胞的影响。结果表明，在A-SrHANF上培养的MG63细胞比在A-HANF上培养的细胞具有更高的成骨相关基因表达。此外，将MG63细胞培养在R-SrHANF和A-SrHANF上，以评估纤维取向对细胞行为的影响。在A-SrHANF上，细胞沿纳米纤维方向排列，具有典型的双极形态，并且比在R-SrHANF上的细胞表现出更高的成骨相关基因表达。因此，羟基磷灰石纳米纤维基质的成分和取向是影响成骨过程的关键参数。
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