Yazdani Morteza, Barta Anita, Berkecz Róbert, Agbadua Orinamhe Godwin, Ványolós Attila, Hohmann Judit
Institute of Pharmacognosy, University of Szeged, Eötvös u. 6, H-6720, Szeged, Hungary.
Institute of Pharmaceutical Analysis, University of Szeged, Somogyi u. 4, 6720, Szeged, Hungary.
Phytochemistry. 2023 Jan;205:113480. doi: 10.1016/j.phytochem.2022.113480. Epub 2022 Oct 22.
Investigation of the chloroform and ethyl acetate extracts of the edible mushroom Pholiota populnea led to the isolation of eight triterpenes, the undescribed natural products pholiols E-K and the known (+)-clavaric acid. HRESIMS and 1D and 2D NMR spectroscopy were employed to determine the structures of the undescribed compounds. The NOESY spectra were used to assign the relative configurations of triterpenes. The isolated compounds were screened for their anti-inflammatory activity on cyclooxygenase (COX-1 and COX-2), and lipoxygenase (5-LOX and 15-LOX) inhibitory assays. Dose-response investigations revealed that lanostane derivatives exhibited moderate 5-LOX and COX-2 inhibitory activities, with pholiol F (IC 194.5 μM against 5-LOX and 439.8 μM against COX-2) the most active among the isolated compounds. Our findings indicated that P. populnea is an abundant source of new bioactive lanostane-type triterpenes.
对食用蘑菇淡黄鳞伞的氯仿和乙酸乙酯提取物进行研究，从中分离出了8种三萜类化合物，即未描述过的天然产物淡黄醇E - K以及已知的(+)-棒酸。采用高分辨电喷雾电离质谱（HRESIMS）以及一维和二维核磁共振波谱法来确定这些未描述化合物的结构。通过核欧沃豪斯效应光谱（NOESY）来确定三萜类化合物的相对构型。对分离得到的化合物进行了环氧化酶（COX - 1和COX - 2）以及脂氧合酶（5 - LOX和15 - LOX）抑制活性的抗炎活性筛选。剂量反应研究表明羊毛甾烷衍生物表现出中等程度的5 - LOX和COX - 2抑制活性，其中淡黄醇F（对5 - LOX的IC₅₀为194.5 μM，对COX - 2的IC₅₀为439.8 μM）是分离得到的化合物中活性最强的。我们的研究结果表明，淡黄鳞伞是新型生物活性羊毛甾烷型三萜类化合物的丰富来源。
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