Department of Obstetrics and Gynecology, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, P.R. China.
Department of Orthopaedic, XiangZhou District Renmin Hospital, Xiangyang, Hubei, P.R. China.
Medicine (Baltimore). 2022 Oct 21;101(42):e31159. doi: 10.1097/MD.0000000000031159.
To analyze the expression and prognostic value of paired-box 8 (PAX8) expression in uterine corpus endometrial carcinoma (UCEC) by bioinformatics. The expression of PAX8 gene in UCEC was analyzed by R language and immunohistochemistry. The correlation between PAX8 expression and clinicopathological features was analyzed by R language. The prognostic factors was analyzed by univariate/multivariate regression. The survival curve of patients was analyzed by Kaplan-Meier Plotter (K-M Plotter). The diagnostic value of PAX8 in UCEC was analyzed by receiver operating characteristic curve, and the relationship between PAX8 expression and methylation was analyzed by Ualcan. The relationship between methylation and prognosis was analyzed by MethSurv database. The expression of PAX8 in cancer tissues was significantly higher than that in normal tissues. The expression of PAX8 was related to clinical stage, age, histological type, histologic grade, tumor invasion and disease-specific survival event. Univariate/multivariate regression analysis showed that clinical stage, tumor invasion, and PAX8 expression were the influence factors of overall survival (OS), while histologic grade and PAX8 expression were the influence factors of disease-specific survival, and patients with low expression had a longer OS. The area under the curve of receiver operating characteristic curve was 0.81 for PAX8 diagnosis of UCEC. PAX8 was hypomethylated in cancer tissue, and patients with hypermethylated PAX8 had a longer OS. The high expression of PAX8 induced by hypomethylation may play an important role in the occurrence and prognosis of UCEC.
通过生物信息学分析配对盒 8（PAX8）在子宫体子宫内膜癌（UCEC）中的表达及其预后价值。使用 R 语言和免疫组织化学分析 UCEC 中 PAX8 基因的表达。使用 R 语言分析 PAX8 表达与临床病理特征的相关性。采用单因素/多因素回归分析预后因素。采用 Kaplan-Meier Plotter（K-M Plotter）分析患者的生存曲线。通过接收者操作特征曲线（ROC）分析 PAX8 在 UCEC 中的诊断价值，并通过 Ualcan 分析 PAX8 表达与甲基化的关系。通过 MethSurv 数据库分析甲基化与预后的关系。PAX8 在癌组织中的表达明显高于正常组织。PAX8 的表达与临床分期、年龄、组织学类型、组织学分级、肿瘤浸润和疾病特异性生存事件有关。单因素/多因素回归分析表明，临床分期、肿瘤浸润和 PAX8 表达是总生存（OS）的影响因素，而组织学分级和 PAX8 表达是疾病特异性生存的影响因素，低表达患者的 OS 更长。PAX8 诊断 UCEC 的 ROC 曲线下面积为 0.81。PAX8 在癌组织中呈低甲基化，PAX8 高甲基化的患者 OS 更长。低甲基化诱导的 PAX8 高表达可能在 UCEC 的发生和预后中起重要作用。
