miR-1246 被认为是一种可能的候选物，可促进水牛（Bubalus bubalis）子宫内膜重塑，从而有利于着床。
miR-1246 is implicated as a possible candidate for endometrium remodelling facilitating implantation in buffalo (Bubalus bubalis).
机构信息
Animal Genomiccs Lab, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal, India.
Department of Biological Sciences, Indian Institute of Science Education and Research, Mohali, India.
出版信息
Vet Med Sci. 2023 Jan;9(1):443-456. doi: 10.1002/vms3.968. Epub 2022 Oct 25.
BACKGROUND
The microRNAs (miRs) secreted by the trophectoderm (TE) cells have recently been implicated in the conceptus-endometrial cross talk during implantation and placentation. These miRs modulate various cellular processes during conception and throughout the pregnancy by regulating the gene expression in the foetal and maternal tissues.
OBJECTIVES
This study was undertaken to elucidate the function of TE secreted miRNAs in the maternal-foetal cross-talk during implantation/placentation in buffalo.
METHODS
The in vitro produced blastocysts were cultured on a cumulus feeder layer for 21 days. The relative expression profiles of a selected panel of miRs was generated using the spent media collected on Days 0, 7, 12, 16, and 21. A custom-designed mirVana™ miRNA mimic was used to transfect the endometrial epithelial cells (EECs) in order to determine the role of miRNA exhibiting highest expression on Days 21 and 21.
RESULTS
The expression of miR-1246 (p < 0.001) and let-7b (p < 0.01) was found to be significantly higher on Day 21 of TE culture in comparison to the control (Day 0). This elevated expression indicated the involvement of these miRs in the maternal-foetal cross-talk. Interestingly, after the transfection of EECs with miRNA mimic for miR-1246 (a novel molecule vis-à-vis implantation), the expression of beta-catenin and mucin1 in these cells was found to be significantly (p < 0.05) downregulated vis-à-vis the control, that is, the IFN-τ primed EECs (before transfection).
CONCLUSIONS
The TE secreted miR-1246 appeared to lower the expression of the endometrial receptivity genes (mucin1 and beta-catenin) which apparently assists the endometrium in preparing for placentation.
背景
滋养层细胞分泌的 microRNAs（miRs）最近被认为在胚胎着床和胎盘形成过程中参与了胚胎-子宫内膜的相互作用。这些miRs 通过调节胎儿和母体组织中的基因表达，调节受孕和整个孕期的各种细胞过程。
目的
本研究旨在阐明水牛胚胎着床/胎盘形成过程中滋养层细胞分泌的 miRNAs 在母体-胎儿相互作用中的功能。
方法
体外产生的囊胚在卵丘 feeder 层上培养 21 天。使用收集的第 0、7、12、16 和 21 天的培养液生成选定 miRNA 面板的相对表达谱。使用定制设计的 mirVana™ miRNA 模拟物转染子宫内膜上皮细胞（EECs），以确定在第 21 天表达最高的 miRNA 的作用。
结果
与对照（第 0 天）相比，发现滋养层细胞培养第 21 天的 miR-1246（p < 0.001）和 let-7b（p < 0.01）的表达显著升高。这种升高的表达表明这些 miRs 参与了母体-胎儿的相互作用。有趣的是，用 miRNA 模拟物转染 EECs 后 miR-1246（与植入相关的新分子），这些细胞中β-catenin 和 mucin1 的表达明显（p < 0.05）下调，与对照（即 IFN-τ 预刺激的 EECs（转染前）相比。
结论
滋养层细胞分泌的 miR-1246 似乎降低了子宫内膜容受性基因（mucin1 和β-catenin）的表达，这显然有助于子宫内膜为胎盘形成做准备。
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