TU Dresden, B CUBE - Center for Molecular Bioengineering, Dresden, Germany.
Cluster of Excellence Physics of Life, Technische Universität Dresden, Dresden, Germany.
Bioessays. 2022 Dec;44(12):e2200149. doi: 10.1002/bies.202200149. Epub 2022 Oct 25.
Outer membrane proteins (OMPs) maintain the viability of Gram-negative bacteria by functioning as receptors, transporters, ion channels, lipases, and porins. Folding and assembly of OMPs involves synchronized action of chaperones and multi-protein machineries which escort the highly hydrophobic polypeptides to their target outer membrane in a folding competent state. Previous studies have identified proteins and their involvement along the OMP biogenesis pathway. Yet, the mechanisms of action and the intriguing ability of all these molecular machines to work without the typical cellular energy source of ATP, but solely based on thermodynamic principles, are still not well understood. Here, we highlight how different single-molecule studies can shed additional light on the mechanisms and kinetics of OMP biogenesis.
外膜蛋白 (OMPs) 通过作为受体、转运蛋白、离子通道、脂肪酶和孔蛋白来维持革兰氏阴性细菌的活力。OMPs 的折叠和组装涉及伴侣蛋白和多蛋白机器的同步作用，这些伴侣蛋白和多蛋白机器将高度疏水性多肽护送至其靶向外膜，使其处于折叠能力状态。以前的研究已经确定了蛋白质及其在 OMP 生物发生途径中的参与。然而，这些分子机器的作用机制以及它们在没有典型细胞能源 ATP 的情况下仅基于热力学原理工作的令人好奇的能力，仍然没有得到很好的理解。在这里，我们强调了不同的单分子研究如何进一步阐明 OMP 生物发生的机制和动力学。
