从尿路感染患者分离的 及 基因的基因检测。
Genotypic Detection of and Genes in Isolated from Patients with Urinary Tract Infection.
机构信息
Department of Medical Laboratory Techniques, Faculty of Medical and Health Techniques, University of Alkafeel, Najaf, Iraq.
Department of Physiology and Pharmacology, College of Nursing, Altoosi University College, Najaf, Iraq.
出版信息
Arch Razi Inst. 2022 Apr 30;77(2):675-680. doi: 10.22092/ARI.2022.357122.1979. eCollection 2022 Apr.
The urinary tract infection (UTI) is a prevalent infection that affects people of all ages. Bacterial agents are the most common causes of UTIs. , , , and other , and () account for a smaller number of infections. These pathogens are transported into the urinary tract from the colonic biotope into dysbacteriosis. Urine samples were randomly collected from 249 outpatients who were suspected of having UTIs. After genital cleaning, 10 mL of urine specimens were collected in a sterilized bowel. Then, the specimens were centrifuged at 2,000 rpm for 5 min and the residue was aerobically incubated with the broth infusion of brain flasks at 37°C for 24 h and then applied with a sterile ring onto blood agar plates and MacConkey agar (OxoidTM). Out of 249 urine samples, the results proved that there were 176 (70.7%) and 51(20.5%) gram-negative and gram-positive bacteria isolates, respectively. However, the results demonstrated that there were 22 (8.8%) urine samples with no growth. In addition, the results showed that eight various antimicrobials are used to treat . In the current study, was treated with eight different antimicrobial agents. The antimicrobial resistance rate for 7 isolates against Cefotaxime, Ceftriaxone, Ciprofloxacin, and Levofloxacin was high for 6 (85.71%) isolates. The results indicated that 6 and 5 isolates had 85.71% and 71.42% antimicrobial resistance against Ceftazidime and Levofloxacin, respectively. Whereas Gentamicin showed a moderate rate of resistance (4 isolates, 57.14%), and Amikacin resistance was found in 5 isolates, accounting for 28.57%. The bacterial isolates had a high susceptibility rate to Imipenem. The gene was found in 6 (85.71%) isolates. However, the recorded data demonstrated that there is no isolate carrying the gene. Among all pathogenic bacteria, was the lowest causative agent of UTI in this study and was highly resistant to most antimicrobials except Imipenem, which was a good antibiotic with 100% sensitivity.
尿路感染（UTI）是一种普遍的感染，影响所有年龄段的人。细菌是引起 UTI 的最常见原因。大肠杆菌、肺炎克雷伯菌、奇异变形杆菌和其他肠杆菌科细菌占较小比例的感染。这些病原体从结肠生物区系进入菌群失调，被运送到尿路。随机采集了 249 名疑似患有 UTI 的门诊患者的尿液样本。生殖器清洁后，用无菌管收集 10 毫升尿液标本。然后，将标本以 2000rpm 离心 5min，沉淀物在脑瓶肉汤中需氧孵育 37°C 24h，然后用无菌环将其涂在血琼脂平板和麦康凯琼脂（OxoidTM）上。在 249 个尿液样本中，结果证实有 176 个（70.7%）和 51 个（20.5%）革兰氏阴性和革兰氏阳性菌分离株，分别。然而，结果表明有 22 个（8.8%）尿液样本无生长。此外，结果表明，有 8 种不同的抗生素用于治疗。在当前研究中，有 7 株分离株对头孢噻肟、头孢曲松、环丙沙星和左氧氟沙星的抗菌药物耐药率较高，为 6（85.71%）株。结果表明，6 和 5 株分离株对头孢他啶和左氧氟沙星的抗菌药物耐药率分别为 85.71%和 71.42%。而庆大霉素的耐药率为中度（4 株，57.14%），阿米卡星耐药 5 株，占 28.57%。细菌分离株对亚胺培南的敏感性较高。该基因在 6 株（85.71%）分离株中被发现。然而，记录的数据表明，没有携带该基因的分离株。在所有致病菌中，在本研究中，奇异变形杆菌是 UTI 的最低致病因子，除亚胺培南外，对大多数抗生素均高度耐药，亚胺培南是一种敏感性为 100%的良好抗生素。
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